BrainMap

Mr. Adrian PIRNAU

researcher - n/a

ResearcherID: B-7093-2011

Curriculum Vitae (18/01/2019)

Expertise & keywords

Homogenous immunoassay technique based on functionalized nanoparticles. Application to detection of pesticide contaminant 2,4-dichlorophenoxyacetic acid from alimentary and environmental samples Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-0402 2012 - 2016

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH

Project partners: INSTITUTUL NATIONAL DE CERCETARE - DEZVOLTARE PENTRU FIZICA SI INGINERIE NUCLEARA " HORIA HULUBEI " - IFIN - HH (RO); UNIVERSITATEA "ALEXANDRU IOAN CUZA" IASI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M (RO)

Project website: http://proiecte.nipne.ro/pn2/138-proiecte.html

Abstract:

Immunological methods such as enzyme linked immunosorbent assay (ELISA) are increasingly becoming important for pesticides residual analysis due to the high specificity of detecting molecules like antibodies. These immunoassay methods are highly specific, sensitive (nanogram or picogram) and accuracy for the detection of low molecular weight contaminants presents in our environment. ELISA is a technique based on the ability of non-labeled antigen (e.g. pesticide) in a specific volume of standard solution or in an unknown sample to compete with a fixed of amount of enzymatic labeled antigen for a limited number of binding sites of a specific binding antibody protein. The objective of the project is to develop a new and innovative immunochemical technique based on functionalized nanoparticles, homogenous enzyme linked immunosorbent assay (HnELISA) technique for detection of pesticide contaminant 2,4-dichlorophenoxyacetic acid (2,4D) from alimentary and environmental samples. 2,4D is one of the most used herbicide in agriculture to control and destroy of the weeds that can affect agricultural crops. The remanence of this organochlorurate compound in alimentary products, transfer and contamination of ground water in the areas where this pesticide is used require the analysis of this chemical in order to establish the contamination level of the alimentary products and the environmental factors (water, soil).
The objective of the project is to develop an immunoassay technique with improved qualities in comparison with traditionally ELISA technique existed on the market for detection of pesticide contaminants from alimentary and environmental factors. Qualitative characteristics as sensitivity, accuracy, stability of the nanoimmunosorbent and low cost per assay are finally taken in account. Development of HnELISA technique would have practical application in monitoring of the pesticide contaminant 2,4D from alimentary and environmental samples.

Bioligand - macromolecule intermolecular interactions as probed by spectroscopic and microcalorimetric techniques Call name: Exploratory Research Projects - PCE-2011 call
PN-II-ID-PCE-2011-3-0032 2011 - 2016

Role in this project:

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Tehnologii Izotopice si Moleculare, Cluj-Napoca

Project partners: Institutul National de Cercetare-Dezvoltare pentru Tehnologii Izotopice si Moleculare, Cluj-Napoca (RO)

Affiliation: Institutul National de Cercetare-Dezvoltare pentru Tehnologii Izotopice si Moleculare, Cluj-Napoca (RO)

Project website: http://itim-cj.ro/PNCDI/idei8/

Abstract:

The study of intermolecular interactions in solutions is of central importance to most chemical and biochemical disciplines. The solution behaviour of bio and macromolecules, their aggregation or intermolecular interactions is relevant for their biological activity. The binding affinity and the molecular recognition capacity can be described by the dissociation constant and the number of binding sites on the macromolecule. Any interaction that interferes with the binding of a bio-molecule to its receptor, such as competitive binding, may affect the pharmacological activity. The purpose of this project consist in the characterization of the molecular binding process between bio-ligands and receptors, using a variety of spectroscopic (nmr, uv/vis and fluorescence) and calorimetric techniques (ITC). Moreover, we intend to investigate the competitive binding process of two ligands to a macromolecular receptor and of two receptors to a bio-ligand. NMR is the major technique used to analyze the interaction between a bio-ligand and a receptor giving useful information about the, stoichiometry, stability, specific and non-specific binding sites. ITC is one of the most powerful and precise technique currently available to characterize the energetics of intermolecular interactions. It allows the binding functions ΔG, ΔH and ΔS to be determined within a single experiment and an accurate determination of the reaction stoichiometry and the dissociation constant.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects