BrainMap

Anisoara Cimpean

- UNIVERSITATEA BUCURESTI

Researcher | Teaching staff | Scientific reviewer | Manager

Web of Science ResearcherID: not public

Curriculum Vitae (07/04/2020)

Expertise & keywords

Novel technology for implants manufacturing from 3D printable reinforced composite filaments for guided bone regeneration Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-1650 2022 - 2024

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA NAŢIONALĂ DE ŞTIINŢĂ ŞI TEHNOLOGIE POLITEHNICA BUCUREŞTI (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation:

Project website: http://www.florinmiculescu.ro/bonegapfill/

Abstract:

Compared to the limited market of products destined for bone reconstructive surgery and the high-patient-risks of current approaches, this project is a necessity to solve the absence of 3D products with optimal geometry, internal architecture and mechanical properties for customized compatibility with natural bone and a rapid repair of defects with variable dimensions. The overall goal of the project is to develop and promote a new reproducible and sustainable manufacturing technology for the products fabrication by 3D printing, using as platform the previously implemented technology for the synthesis of hydroxyapatite derived from bovine bone biogenic resources and the project team`s experience in the field. Composite filaments with printable features will be obtained based on natural hydroxyapatite and two polymers, one of which will be of natural origin. Also, superior and adaptable mechanical characteristics will be ensured by reinforcing the ceramic matrix with multi-layer graphene-based materials. Further, the filaments will be used for 3D printing of products with regular and random internal architecture (based on a new STL file developed within the project). Afterwards, the products will be tested as to evaluate their performance as potential bone replacements. In this regard, a patent application will be filed. The proposed topic is new and challenging for the project team, but all the premises are fulfilled through the team`s synergy and previous research experience. The concept and experimental testing of the possibility of embedding naturally derived ceramic particles into a polymer matrix of natural origin were also demonstrated and reported by the team members as viable for achieving the project objectives.

Multicomponent hydrogel with hybrid structure obtained in situ by irradiation technology for the malignant melanoma therapy Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală - PD-2021
PN-III-P1-1.1-PD-2021-0552 2022 - 2024

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO)

Affiliation:

Project website: http://hydrocan.ale.inflpr.ro/

Abstract:

The project aims to develop a multicomponent hydrogel with elastic and hybrid structure obtained from polymeric mixtures consisting of chitosan and collagen, synthetic polymers and doxorubicin (DOX), to be used in the treatment of malignant cancers of the skin, as a new alternative for topical administration of chemotherapeutic drugs (CM). The hydrogel will be obtained in a single technological step without the addition of toxic chemical reagents, by crosslinking with electron beams (e-beam).

The cytotoxic and anti-proliferative effects of chemotherapeutically loaded hydrogels (CM) capable of delivering an amount of DOX equivalent to IC50 will be estimated by performing LIVE / DEAD and CCK-8 cell viability / cytotoxicity tests and compared to the cellular response to hydrogels. without CM. All these studies will be performed comparatively on melanoma cells and fibroblasts (as a non-tumor cell line).

The proposed hydrogel will be sterile and moderately crosslinked, properties provided by e- beam treatment, biocompatible and biodegradable, with adequate mechanical properties and rate of absorption and degradation controllable in physiological environments and environments similar to the tumor site. To ensure the targeted therapeutic effect, it will support cell viability and proliferation and facilitate the release of chemotherapeutic drugs (CMs) in a controllable manner.

Integrated development project for advanced medical treatment technologies Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0728 2018 - 2021

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE MEDICO-MILITARA „CANTACUZINO” (RO); INSTITUTUL DE BIOCHIMIE (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA PITESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://teramed.inflpr.ro/

Abstract:

In order to increase community’s quality of life, the aim of the project proposal entitled “Integrated development project for advanced medical treatment technologies” (TERAMED) is to develop novel technologies with respect to the treatment of osseous and cutaneous conditions and oncological disorders. Given our experience in healthcare research and the current requirements of multidisciplinary and interinstitutional collaboration towards the personalized treatment purpose, the TERAMED project aims genuine synthesis and processing of biomaterials, but also functional and therapeutic evaluation relevant for clinical trials. The main objectives of the “Medical devices functionalized by laser technologies and alternatives for enhanced osseous integration and regeneration” subproject are to design and produce inorganic, composite or hybrid coatings for superior osteoconductive and osteoinductive performances of titanium-based implants. Smart wound patches and polymeric gels functionalized with antimicrobial and wound healing biomolecules incorporated within micro- and nanoparticles constitute the purpose of the “Medical devices (patches and gels) based on composite biomaterials obtained by laser, plasma and radiation technologies and alternatives for enhanced healing of cutaneous injuries” subproject. The “Technologies based on magnetic triggered nanostructures for oncological therapy: early diagnosis and targeted treatment” subproject aims the development of multifunctional medical devices for specific and selective diagnosis and treatment of breast cancer and melanoma. The general impact of the TERAMED project ensues from the beneficial conjunction of the clinical potential of the proposed medical devices, the feasible technological transfer and the economic advantages of interinstitutional collaboration.

Advanced Innovative approaches for predictable regenerative medicine Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0782 2018 - 2021

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE IN DOMENIUL PATOLOGIEI SI STIINTELOR BIOMEDICALE "VICTOR BABES" (RO); INSTITUTUL ONCOLOGIC PROF.DR.I.CHIRICUTA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/65pccdi-2018/

Abstract:

Considering that HEALTH is one of the main areas of public priority, the approach of interdisciplinary researches with applicability in Regenerative Medicine can significantly contribute to improving the quality of life of patients with tissue defects. The aim of the project is to create a consortium with complementary research experience in the field of regenerative medicine, which will efficiently use the human resource and modern research infrastructures newly created for the implementation of innovative technologies, with the aim of developing and transferring the results to the economic environment. The project aims to develop new technological products in the form of biocompatible biomaterials designed for bone reconstruction (P1), nerves (P2), soft tissues (P3) and breast reconstruction after tumor resection (P4).

Functional Nanostructured Implant for Bone Fixation Call name: P 3 - SP 3.2 - Proiecte ERA.NET - COFUND
COFUND-ERANET MANUNET III-BoneFix 2019 - 2020

Role in this project:

Coordinating institution: UNIVERSITATEA "DUNAREA DE JOS"

Project partners: UNIVERSITATEA "DUNAREA DE JOS" (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.bonefix.ugal.ro/

Abstract:

The project objective is to obtain orthopaedic implants from a new biomaterial (Gum type alloy) nanostructured by Severe Plastic Deformation having antibacterial nanocoatings. The project provides advanced solution with powerful societal impact by developing temporary implants, new compression staples with multidirectional gripping, able to ensure better fixation of bone fragments especially at the multiple fractures. For achieving project goals, scientific activities will be developed concerning the following: design of the alloy composition; alloy synthesis; study of the alloy deformability; thermomechanical alloy processing by High Speed High Pressure Torsion method; complex characterization of the ascast/processed alloy; antibacterial nanostructured coatings deposition; in vitro advanced characterization by electrochemical studies to asses the corrosion behaviour of the alloy and of the coatings; in vitro characterization by biological cellular response analysis; finally, implants design and execution. In addition, the project aims to demonstrate the applicability of the research results and validation both manufacturing and characterizing technologies on a number of test implants.

Spaced titania nanotubes as platforms for drug delivery and bone regeneration Call name: P 4 - Proiecte de Cercetare Exploratorie
PN-III-P4-ID-PCE-2016-0691 2017 - 2019

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: https://unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte-de-cercetare/proiecte-cu-finantare-nationala/pce-55-2017/

Abstract:

In spite of the vast development and clinical use of titanium (Ti) based metallic implants and of their gold standard status for dental/orthopedic implants, in some cases Ti implants are encapsulated in vivo by fibrous tissue and show a lack of osseointegration which can finally lead to implant failure. To improve implant viability and its biologic performance, nanotechnology and local drug delivery strategies hold certain advantages through the modification of implants’ surface. In this context, the main objective of this project is to evaluate novel platforms for bone regeneration and drug delivery applications. These platforms consist of spaced titania nanotubes modified Ti (TNT) with varying characteristics, loaded with simvastatin (with and without PLGA coating) as a model drug known to promote bone tissue formation. The team participating in this project, having a rich expertise in the field of biocompatibility and regenerative medicine, will be involved in the fulfillment of the following scientific objectives: 1) development of an in-depth understanding on the effects of spaced TNT on cells relevant to bone regeneration; 2) in vitro evaluation of the potential of spaced TNT to load bioactive agents (simvastatin) and to release them in a controlled manner; 3) assay of the efficiency of spaced TNT as platforms for drug delivery and bone regeneration in in vivo animals models. All the data obtained will be integrated in order to identify the best features of the nanostructured material for targeted application. The original and innovative approaches of this project are expected to contribute to the creation of fundamental knowledge on the influence of spaced TNT on macrophage and osteoblast cells and to have a high impact on the improvement of quality of life of those who need dental and orthopedic implants by a potentially faster recovery and reduced risk of failure or side-effects.

Porous and nanostructured magnesium biodegradable alloy implants, with bioactive nanocoatings, controlled degradation and improved osseointegration Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-MANUNET II -BioImplantMag 2017 - 2019

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); UNIVERSITATEA BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE- DEZVOLTARE PENTRU MICROTEHNOLOGIE - IMT BUCURESTI INCD (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.mdef.pub.ro/research/BioImplantMag/ro/index.html

Abstract:

The project provides an improved solution, with societal impact, by developing temporary implants of metallic materials based on magnesium alloys that eliminate the costs of surgical re-interventions required to remove the "classic" temporarily implants with advantages for the comfort of the patient and for health costs. For achieving project goals, scientific activities will be effectuated concerning the following: design of the alloy composition; alloy synthesis by melting in furnace; study of the alloy deformability; thermo-mechanical alloy processing by extrusion and by SPD; complex characterization of the as-cast and mechanical processed alloy; in-vitro advanced characterization by electrochemical studies and hydrogen evolution tests and by biological response analysis; implants design and execution. It will be demonstrated the applicability of the research results and will be validated the technologies obtained through manufacture and characterization of a lot test implants.

Advanced dental implants from a high biocompatible beta Ti alloy with functionalized surface Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-MANUNET II -BioTiDent (3) 2017 - 2018

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://prof.unibuc.ro/prof-habil-anisoara-cimpean/manunet-31-2017/

Abstract:

The project “Advanced dental implants from a high biocompatible beta Ti alloy with functionalized surface” has the objective to design an innovative dental implant by means of the development of a new superelastic beta Ti alloy with superior mechanical properties. Besides, implants can be provided with bioactive and antibacterial properties by their surface functionalization with multifunctional coatings composed by oxides doped with Ca, P and fluorides, using plasma electrolytic oxidation technology. These biocompatible coatings will also supply to the implants better corrosion and wear resistance due to their excellent protective properties. As a result of the project, a more resistant dental implant in terms of mechanical, corrosion and tribological properties will be obtained with better osseointegration and antibacterial features, which will result in an improved quality of life for the patient.

New technology for the synthesis and manufacturing of biomimetic implants derived from biogenic resources used for bone reconstruction surgery Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0892 2017 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.florinmiculescu.ro/bioimms/

Abstract:

The overall goal of this project is to develop and implement a new complex and reproducible synthesis technology of biomimetic ceramic materials (hydroxyapatite and tricalcium phosphate, in different proportions) derived from marble and seashell precursors and their performance assessment as potential bone substitutes in restorative and reconstructive surgery. This project proposes the synthesis of a mineral phase presenting mechanical properties at least similar to those found at bone structural arrangement level. Once the biomechanical requirements are met, the biomimetic products will be tested through in vitro standardized methods to assess their biological performance. The ultimate goal of this project is to achieve biomimetic implants (compact and porous products) with custom features related to the mechanical requirements for an optimal biofunctionalization. In this regard, one patent application will be submitted, but it will not be funded through this project.

This project aims to develop, implement and promote a ceramic biomaterial known as biogenic hydroxyapatite. This is expected to lead to a new concept of biomimetic implant with improved features, for a rapid and complete resolution of all sizes bone defects.

The proposed subject is new and challenging for the project members, but all premises (for congent result achievement) are met through the sinergy and extended collaboration of both teams. The initial TRL level 2 was gradually established through the expertise of the project team. The project starts from conceptual approaches adopted from some studies and applied to the selected precursors. Experimental testing of research hypothesis will lead to an improved synthesis route. The appropiate manufacturing methods will be assessed for both types of biomimetic products (TRL level 3). Once the best experimental route is established, a comission constituted by independent experts will validate the proposed empirical model (TRL level 4).

Biomimetic 3D-printed porous scaffolds for bone tissue engineering Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0952 2017 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte/proiecte-cu-finantare-nationala/ped-127-2017/

Abstract:

The project BioGraftPrint approches an experimental and demonstrative research seeking the development of novel strategies for improving bone healing by means of advanced materials, methods and technologies. In line with this, the project aims at developing novel Zr2+/Sr2+-doped hydroxiapatite/collagen/chitosan-synthetic bone grafts with well-defined and controllable architecture by means of a new generation technique, namely 3D printing, which ensures high precision and customized production. In view of implementing the demonstrator model, the project begins from a conceptual level (TLR2), represented by the compositional and morphological design of the product, which will be materialized by initiation of the active process of research and development involving material synthesis and characterisation, as well as preliminary in vitro and in vivo laboratory studies (TLR3). The successful accomplishment of relevant activities will be assured by a close synergistic cooperation between project partners with complementary expertises in the fields of bioengineering, material science, biology and medicine. Their previously cooperation in many national/international projects concerning biomaterials development represent a starting point for the further project implementation. Thus, to satisfy the project objectives, UPB will be responsible with the synthesis and characterization of the new graft materials, while UB will be involved in the in vitro biological evaluation and in vivo testing of the novel 3D-printed materials. Original contribution of each partner will be disseminated in order to generate novel knowledge regarding particular relations between material composition/phisical/mechanical characteristics and biocompatibility/host tissue responses. By achieving these tasks, the current project will represent a step further towards the development of new bio-inspired materials which are expected to increase life expectancy of patients with bone related traumas.

Enhancement of bone defect repair by mussel-inspired TiO2-based nanostructures functionalized with bioactive phytomolecules Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1372 2017 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://prof.unibuc.ro/prof-abil-anisoara-cimpean/ped-149-2017/

Abstract:

Despite the vast development and clinical use experienced by the metal implants, such as titanium (Ti), these are far from optimal. The use of bioactive coatings is one of the most promising strategies to promote appropriate surface-cell interactions at the implantation sites. In this context, the project scope is to immobilize bioactive phytomolecules of Herba Epimedii (EpFs) with osteoprotective effect on Ti surfaces nanostructured with TiO2 nanofibers and nanotubes via polydopamine (PDA) and nitrodopamine (NDA) films as intermediate layers, and to investigate how such surface functionalization would affect the osteoblast behaviour and inflammation responses of RAW264.7 cells. Finally, in vivo biological performance of the developed coatings will be assessed.

The two academic partners with complementary expertise participating in this project will be involved in the fulfillment of the following scientific objectives: Obtaining of nanostructured Ti/TiO2 surfaces, deposition of PDA- and NDA- based films and EpF-immobilization; In-depth characterization of the developed films deposited on Ti/TiO2; Evaluation of in vitro biological performance of the elaborated films; Obtaining of the demonstrator product (EpF functionalized PDA/NDA-coated Ti/TiO2 pins); Development of an in vivo experimental model for osseointegration of the demonstrator product. Thus, our starting point is a TRL 2 which represents a concept – DA is a bioadhesive. We have already verified this concept using a well-established procedure and now we take the advantages of this property for bonding EpFs and to test a related bioadhesive (NDA) from the same class. After implementation of the starting concept, this will be developed and validated from next points of view: physico-chemical surface properties and biological performance in order to prove the bioactivity and feasibility of the coating and functionalization technologies which correlates with a TRL 3.

Innovative surface design to extend the life and safety of dental and orthopedic implants Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0910 2017 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte/proiecte-cu-finantare-nationala/ped-97-2017/

Abstract:

NovSurf addresses a critical health issue, namely the increasing incidence of dental and orthopedic implant revisions due to implant associated infection and inflammation, by proposing a novel approach based on development of a surface topography of nanochannelar type on titanium implants by anodization, on which zinc oxide nanoparticles will be incorporated as antimicrobial agents. The newly designed surface is expected to reduce the susceptibility of titanium implants to microbial colonization, to enable the decontamination of implants if infection occurs and to support bone healing around the implant. In-depth characterization of physical properties, chemical composition and biological activity of modified titanium surface is envisaged. In vitro studies will evaluate its antimicrobial and anti-biofilm properties, its bioactivity, and inflammatory potential. Preliminary in vivo evaluation of the extent of bone regeneration after insertion in rats femur of surface modified titanium pins will provide efficacy data insuring the proof of concept by the end of the project. The project is built on two pillars of expertise afferent to the institutions involved, namely the strong background in the electrochemical deposition of TiO2 nanostructures on implant materials and their characterization at University Politehnica of Bucharest, and the excellent capabilities in assessment of microbial and cell-biomaterial interactions at University of Bucharest. The team gathers researchers with complementary expertise acting in a synergistic manner in order to solve an important health issue related to implant failure. If our expectation will be fulfilled, the findings may form a good foundation for further potential clinical validation of Ti-based implants with this innovative surface design as ready products for clinical applications.

Orthopaedic implants obtained from multifunctional "Gum" alloys Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-1643 2014 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA BUCURESTI (RO); TEHNOMED IMPEX CO S.A. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.mdef.pub.ro/research/IMPLANTGUM/ro/index.html

Abstract:

The use of more sophisticated and more expansive biomaterials for medical devices (as will be ours) practically does not affect the implantation expenditure, because the total involved cost is essentially determined by the surgery and patient’s treatment after implantation. Moreover, the faster osseointegration, less stress shielding avoiding bone atrophy, guarantee implantation success making longer the implant life (over 20 years instead of actually 10 years), improving the patient comfort and also reducing the costs for drugs and reimplantation. The project objective is to obtain orthopedic implants from a new biomaterial, with advanced properties, able to eliminate as many possible causes that lead to surgical intervention.

A group of special beta Ti alloys with unique physical-mechanical properties and large range of possibilities to be used in medical and other applications are “Gum” alloys. From 2003, the development of these alloys acquired a large scientifically and technical interest, because of their special properties. Their composition belongs to beta-type Ti alloys and is basically expressed as Ti (Ta, Nb, V) + (Zr, Hf, O). “Gum” alloys exhibit excellent mechanical multi functionality at room temperature: an ultra-low Young’s modulus (Young’s modulus 60-70 GPa) and a non-linear elastic behaviour, an extended elastic limit, ultra high strength (> 1 GPa), superplastic-like deformability, Invar-like thermal expansion, and Elinvar-like thermal dependence of the elastic modulus. The mechanical properties of the alloy investigated in the present project, a “Gum” alloy type, superior to other materials on the market, make possible to obtain medical devices with considerably increased lifetime, which will lead benefit regarding the patient's life quality and economic advantages.

The enhanced characteristics of the proposed implant biomaterial are: (1) high biochemical compatibility, demonstrated through SBF corrosion and citotoxicity tests of both bioalloy (containing only non-toxic elements); (2) high biomechanical compatibility, demonstrated through the obtained special advanced properties of the “Gum” alloy (improved mechanical properties: tensile strength >1000 MPa; super-elasticity; superplasticity permitting cold plastic working close to technical limit of 99.9 % with no work hardening at room temperature; near zero linear expansion coefficient; a low, constant elastic modulus, close to those of human bone 30–40 GPa).

The needs in biomaterials/implants, at the world level, do not stop growing. The load-bearing implants market (representing 20-25% form implantable devices market) including a significant part of orthopedic and dental prosthetics, dental crowns, implants for maxillofacial surgery, artificial limbs, fixtures, is a large one, estimated at a few billions €. This situation creates favourable conditions for the application of research results at industrial level.

The project has high success chances because its outcomes are two new products necessary on the market and their manufacturing technologies that can be implemented at two industrial agents in Romania that have technical and technological capacity to achieve these products.

Next generation of orthopaedic implants based on new Ti bioalloy functionalized with hybrid biomimetic coatings Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-1994 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA BUCURESTI (RO); INSTITUTUL DE BIOCHIMIE (RO); TEHNOMED IMPEX CO S.A. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://ppam.inflpr.ro/ORTHOBIOMIM.htm

Abstract:

The main objective of ORTHO-BIOMIM project is to obtain competitive and original products, namely advanced orthopaedic implants based on a new Ti-Ta-Nb alloy, which are functionalized by laser based technique (Matrix Assisted Pulsed Laser Evaporation) with complex hybrid biomimetic coatings (Lactofferin, Hydroxyapatite, Polyethilenglycol-Polycaprolactone synthetic copolymer functionalized with methyl ether group, Lf_HA_PEG-PCL-Me). The proposed approach relies on applied experimental research involving strongly interdisciplinary domains (i.e. material science, mechanics, laser physics, chemistry, microbiology, biomedicine), combined with industrial research activities, development.

The strategic key points for obtaining orthopaedic implant with enhanced characteristics within ORTHOBIOMIM project are:

• New and highly biocompatible Titanium (Ti)–based alloy containing non-toxic components (Ti-Ta-Nb) obtained by levitation technique with enhanced properties (low corrosion, mechanical resistance, elastic modulus etc.) working as implant body;

• Improved mechanical characteristics of the alloy obtained by its thermo-mechanical processing;

• Alloy biofunctionalization with complex hybrid coatings having multiple functionalities (biodegradable, bioresorbable, increased capacity osteogenic, osteoconductive and osteoinductive, anti-inflammatory ) by laser methods (MAPLE).To mimic the process of osseointegration, these coatings will be composed of natural biodegradable compounds, namely lactoferrin (Lf) and hydroxyapatite (HA), and a antifouling synthetic copolymer functionalized with methyl ether PEG-PCL-Me);

• Highly flexible methods for obtaining alloy processing and functionalization, allowing control defined composition, chemistry and surface topography when both alloy and the coating / film hybrid biomimetic functional.

The implications anticipated within ORTHOBIOMIM project development are related to major scientific benefits by increasing national competitiveness and visibility in research, development and innovation, but also to social and economic benefits . In the social context, in addition to the involvement of a large number of youth in the project and creating jobs, a very important benefit provided by this project is given by the envisaged faster osseointegration and long lifespan of the implant. This might be of crucial importance for those patients whose health status and life will be enhanced through the use of new medical implants by reducing risks of infection and thus reduce costs for medication or those necessary to replace a failed implant.

By the proposed objectives and approach, the project is aligned with the main points defined Partner Program, which promotes the applied inter and trans-disciplinary research in micro and nanostructured advanced materials for applications in medical field.

New nanostructured multifunctional coatings for orthopaedic implants Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0473 2014 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); TEHNOMED IMPEX CO S.A. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.unibuc.ro/n/cercetare/proiecte/PCCA_2532014.php

Abstract:

The materials used for internal fracture fixations and joint replacements are all currently made of metals. However, metallic implants suffer from two shortcomings, one being the poor interfacial bonding between the metallic surface and surrounding bone, and the other the higher rigidity of metallic implants than that of natural bone. Additionally, complications associated with implants due to bacterial-induced infections arise frequently. The implant anti-bacterial function set additional challenges for implant development besides the requirements for osteoblast fixation, mechanical, and biomechanical constraints, consequently in many cases almost incompatible demands have to be met. For osseointegration it is important to promote osteoblast fixation and to avoid bacterial adhesion at the same time. But, cells and bacteria respond differently to implant surface parameters and this gives an opportunity to look for an optimal solution. The present life expectancy of metallic implants is estimated at 10-15 years. Taking into account the ageing of the population, a second implantation becomes more and more often necessary. Surgical revision, however, can be twice as expensive as the primary operation and may lead to significant complications, including infection, deformity, pain, and loss of mobility. This research project is proposed to adress these issues.

A new metallic material (low elastic modulus titanium alloy) with two types of nanostructured coatings, titanium oxide nanotubes and carbon nanowalls, respectively, will be designed, developed and investigated for optimal implant fixation through innovative integration of material science, physics, chemistry and life science. Cerium oxide nanoparticles will be immobilized by electrodeposition to provide the coatings with antibacterial properties. This new beta-titanium alloy along with the new coatings will impart to orthopedic implants the excellent corrosion resistance, adequate strength, enhanced mechanical compatibility, and good bioactivity for promoting bone tissue regeneration and fixation of implants, and to prevent bacterial infections, offering an innovative solution to all of the issues faced by the present metallic implants.

Functionalized surfaces will be fully characterized for physical properties, chemical composition and biological activity. In vitro biological studies will include adhesion, proliferation and differentiation of osteoblasts, assessment of inflammatory cytokines, nitric oxide release and foreign body giant cell formation in macrophage cultures. In parallel Staphylococcus aureus and Escherichia coli adhesion and biofilm formation on experimental titanium surfaces will be analysed. Collation of all the data will be performed to select from the alternatives the optimal coating. As proof of concept, the fabrication of orthopaedic implant demonstrator is envisaged.

To achieve the research goals, nine work packages have been established, and a research team with all of the requisite expertise has been formed. We firmly believe that the synergism of this research team will allow us to successfully conduct this multidisciplinary project and push the frontier of the field of orthopedic biomaterials.

The results of this project will have favorable social and economic impacts on society because the quality of patient life using the new implants could be improved greatly. Moreover, a reduction in the need for revision surgery could translate into reduced health care costs. The broad impacts of this program will also be evident in our strong commitment to human resource development.

Therapeutic strategies for enhancing bone healing response by using bioactive coatings for bioabsorbable magnesium-based implants Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-2119 2014 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA DE STIINTE AGRONOMICE SI MEDICINA VETERINARA (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); TEHNOMED IMPEX CO S.A. (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.unibuc.ro/prof/cimpean_a/pcca-195-2014/

Abstract:

During last decade, orthopedic implants have attracted an increasing interest for research and clinical applications but still can be said that they are far from being optimal. Current metallic implanted biomaterials remain permanent in the body and the long-term exposure of these non-degradable implants significantly increases the risk of complications such as chronic inflammation and associated foreign body reaction. The implant encapsulation represents one of the major drawbacks of these implants. Besides, several implantable devices are used to achieve a temporary function and a second procedure to remove them is often difficult. The current research is focused on investigating the application of bioresorbable metals, which are expected to support the healing process of the damaged tissue and to degrade further as the functional regenerated tissue is formed. Magnesium (Mg)- based alloys represents o novel class of biodegradable biomaterials with high specific strength and biocompatibility able to achieve a temporary function making possible the avoidance of the costly second replacement. However, the side effects such as hydrogen gas bubbles formed around the implant and a localized pH change, generated by extremely high degradation rate of magnesium in body fluids, can negatively impact on its host-tissue integration.

In this context, the aim of this project is to develop a new biotechnological strategy to overcome the issues previously mentioned and improve the osseointegration capacity of magnesium-based implants. Therefore, a novel coated alloy based on MgCaMnZr system will be developed and investigated by innovative technologies and techniques to obtain a controlled dissolution rate and to reduce the hydrogen gas production.

This material will be thermomechanically processed to allow its structural adjustment and to meet the expected properties. Moreover, the coatings based on cellulose will be functionalized with resveratrol or sericin, two biomolecules recently characterized for their beneficial effects on bone regeneration. We are expecting that the selected novel Mg-based alloy along with the developed coatings will protect the alloy slowing down its rate of degradation, providing the implant with the desired strength in the initial period of healing process and avoiding side effects associated to its rapid degradation. Furthermore, the functionalization of these coatings is expected to accelerate the healing process and the osseointegration of the implant. The project approaches an experimental multidisciplinary research seeking significant advancement in more than one field of science (i.e., biotechnology, material science, medicine etc), involving a qualified research team with specific skills and expertise. Thus, complex in vitro biological investigations consisting of interface studies will be performed in order to select the most suitable alloy formula for the targeted application and further to establish its in vivo biological performance on experimental animal models. The selected samples will be implanted as prototypes in Wistar rats and small domestic animals for in vivo monitoring of host tissue response and defect repair. The developed material and the demonstrator product will be also investigated to evaluate the physico-chemical properties. All the data obtained will be integrated in order to identify the best material’s features for targeted application. The original and innovative biotechnological approach of this project is expected to have an important impact on health related life standard and contribute to increasing life expectancy. We also hope into a positive impact of the project on the health care-associated costs, educational system and human resources.

Developing new graphene-polymer composites biomaterials for scaffold fabrication with applicability in bone repair by coupling multiscale molecular modelling and experiments Call name: Joint Applied Research Projects - PCCA-2011 call, Type 1
PN-II-PT-PCCA-2011-3.1-1538 2012 - 2016

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA "DUNAREA DE JOS" (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA DE VEST "VASILE GOLDIŞ" ARAD (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website: http://www.tsocm.pub.ro/cercetare/POLYGRAPH/

Abstract:

The main aims of this project are interdisciplinary efforts using complex computational tools of materials modelling and advanced experimental techniques for the knowledge-based design of novel, improved biopolymer-graphene scaffolds for bone repair. The key functionality of these materials is both physical and chemical cues to direct cells organization, growth, and differentiation in the process of forming functional tissue. The aim is pursued by developing and extensive application of multiscale computer-aided molecular design complemented with computer-assisted evaluation of the end-use performance of the materials in question. The resulting outputs will constitute the doorstep toward the fabrication of the biopolymer-graphene biomaterials of potential commercial values. This goal is achieved by the development of a complex protocol based on advanced experimental techniques. A method for graphene synthesis and modification by plasma treatment in order to introduce different chemical groups on the surface and graft peptides or proteins capable to facilitated cells adhesion, growth, and tissue remodelling will be establish. The end-use performance of these materials depends on several crucial factors such as graphene surface properties, polymer nature, and the methods used for biomaterial synthesis. On the frame of the experimental activity the research will be focus on modulating synthesis parameters in order to obtain a material suitable for bone repair scaffold. Extensive characterisation of biomaterials surface and bulk by physical-chemical techniques will be employed. The ultimate aim of present project should be the in vitro assessment of biocompatibility in terms of cellular morphology, adhesion, viability and proliferation, and the evaluation of differentiation potential of the new elaborated scaffolds. These studies will be complemented with in vivo assay regarding bone reconstruction with biopolymer-graphene scaffolds.

In vitro effects of beta-titanium alloys on biological behavior of macrophages and their cross-talk with osteoblasts Call name: Exploratory Research Projects - PCE-2011 call
PN-II-ID-PCE-2011-3-0737 2011 - 2016

Role in this project:

Coordinating institution: Universitatea din Bucuresti

Project partners: Universitatea din Bucuresti (RO)

Affiliation: Universitatea din Bucuresti (RO)

Project website: http://www.unibuc.ro/prof/cimpean_a/Proiect-PCE-188-/

Abstract:

The project aims to elucidate the modulation of macrophage function and of the cross-talk between macrophages and osteoblasts by new beta-titanium alloys which are based on titanium and nontoxic alloying elements such as Ta, Nb/Zr. For comparative purposes, a commercial Ti-6Al-4V alloy, will be also investigated. In the first stage, we will investigate if the macrophages cultured in contact with the tested substrates display differences regarding cell viability, proliferation, apoptosis, cytoskeleton and adhesive structures (focal contact, podosomes) which may be important for the interpretation of macrophage function in chronic inflammatory responses. Next, we will evaluate macrophage cytokine, chemokine and matrixin expression at transcriptional and translational levels. The proposed project is an innovative approach which will bring new knowledge on signaling pathways (such as NFκB and MAP-Kinase) that could be activated by various Ti alloy compositions and on material effects upon the cross-talk between macrophages and osteoblasts using a Transwell co-culture model. This cross-talk will be studied in terms of modulation of: i) soluble factors involved in bone turnover (RANKL, OPG); ii) osteogenesis process (alkaline phosphatase activity, mineralization process); iii) macrophage and osteoblast cytokine production. These studies can conduct to the development of cellular models for in vitro assessment of the inflammatory response to osteoprothetic materials.

NEW CONCEPTS AND STRATEGIES FOR THE DEVELOPMENT OF KNOWLEDGE OF NEW BIOCOMPATIBLE STRUCTURES IN BIOENGINEERING Call name: Complex Exploratory Research Projects - PCCE-2008 call
PN-II-ID-PCCE-2008-0248 2010 - 2013

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DIN BUCURESTI-DEPARTAMENTUL DE BIOCHMIE SI BIOLOGIE MOLECULARA

Project partners: UNIVERSITATEA DIN BUCURESTI-DEPARTAMENTUL DE BIOCHMIE SI BIOLOGIE MOLECULARA (RO); INSTITUTUL DE BIOLOGIE SI PATOLOGIE CELULARA NICOLAE SIMIONESCU-LABORATORUL DE CELULE STEM SI TERAPIE CELULARA (RO); UNIVERSITATEA POLITEHNICA BUCURESTI-FACULTATEA DE CHIMIE APLICATA SI STIINTA MATERIALELOR (RO); UNIVERSITATEA POLITEHNICA BUCURESTI-CENTRUL NATIONAL DE CONSULTANTA PENTRU PROTECTIA MEDIULUI (RO); INSTITUTUL DE CHIMIE FIZICA ILIE MURGULESCU (RO); UNIVERSITATEA BABES-BOLYAI CLUJ-NAPOCA, CENTRUL DE BIOMATERIALE, INSTITUTUL DE CERCETARI EXPERIMENTALE SI INTERDISCIPLINARE (RO); INSTITUTUL DE CERCETARE-DEZVOLTARE PENTRU CHIMIE SI PETROCHIMIE BUCURESTI (RO)

Affiliation: UNIVERSITATEA DIN BUCURESTI-DEPARTAMENTUL DE BIOCHMIE SI BIOLOGIE MOLECULARA (RO)

Project website: http://www.pcce248.weebly.com

Abstract:

IN TISSUE ENGINEERING (TE) THE COMBINED KNOWLEDGE FROM BIOLOGY AND ENGINEERING

IS DIRECTED TOWARDS THE POSSIBILITY TO RESTORE LOST OR DAMAGED TISSUE. THE

GENERAL AIM OF THIS PROJECT IS TO CONSOLIDATE A CROSS-DISCIPLINARY TEAM OF

COLLABORATING INVESTIGATORS TO CARRY OUT SOME CELL-SUPPORT CONSTRUCTS (CSC)

WITH POSSIBLE APPLICATIONS IN REGENERATION/REPAIR OF SOFT AND HARD TISSUES AND IT

DOES NOT ASSUME PRE-CLINICAL AND CLINICAL TRIALS. THIS APPLICATION CONTAINS THREE

SPECIFIC OBJECTIVES: 1- OBTAINMENT OF NEW SUPPORT 3-D STRUCTURES DESIGNED TO

CULTIVATE OSTEOBLASTS AND HUMAN MESENCHYMAL STEM CELLS (HMSC) TO OBTAIN CSCS

WITH CHARACTERIZED ARCHITECTURE AND MECHANICAL PROPERTIES, USEFUL IN BONE TISSUE

ENGINEERING; 2 - DEVELOPMENT OF REGENERATION STRATEGIES OF ADIPOSE TISSUE BY

IMPLANTATION OF PREADIPOCYTES IN 3-D HYDROGEL SCAFFOLDS, THAT MIMIC

EXTRACELLULAR MATRIX, DESTINED TO THE RECONSTRUCTION OF SOFT TISSUE DEFECTS

(SEVERE TRAUMAS, DEEP BURNS OR TUMOR RESECTIONS) AND 3 - STUDY OF THE EFFECTS OF 3-

D CULTURE AND GROWTH FACTORS ON THE CHONDROGENIC DIFFERENTIATION OF HMSC CELLS

TO OBTAIN SOME INVESTIGATION MODELS OF THEIR POTENTIAL IN CARTILAGE TISSUE

REGENERATION. THE PROJECT PRESENTS VIABILITY, INNOVATION, COMPLEXITY AND

INTERDISCIPLINARY EXCHANGE BECAUSE: 1 - IT IS A CONSORTIUM WHICH CONSISTS FROM

PARTNERS WITH COMPLEMENTALLY COMPETENCES WHO ENGAGE TO ACT AS A UNITY IN THE

FOLLOWING FIELDS: CELLULAR AND MOLECULAR BIOLOGY, CHEMISTRY AND PHYSICS OF

MATERIALS, ENGINEERING SCIENCES – IN ORDER TO GET ALL OBJECTIVES; 2 - THE PARTNERS

ARE STAFFS WITH A GOOD, STRONG REPUTATION ON THEIR FIELD, AND THEY HAVE THE

NECESSARY MANAGERIAL EXPERIENCE AS WELL AS THE HUMAN RESOURCES AND PERFORMING

EQUIPMENTS AND 3 - THE PARTNERSHIP WAS PARTIALLY CONSOLIDATED DURING PREVIOUS

COLLABORATIONS AND WHICH SUSTAIN IT. ACCOMPLISHMENT OF THE OBJECTIVES OF THIS

PROJECT CONSTITUTES A SCIENTIFIC CHALLENGE FOR ANY SCIENTIFIC TEAM AROUND THE

WORLD.

Call name: Premierea obtinerii atestatului de abilitare - Competitia 2015
PN-II-RU-ABIL-2015-2-0148 2015 -

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO)

Affiliation: UNIVERSITATEA BUCURESTI (RO)

Project website:

Abstract:

BIOcompatible Medical IMplants Elaborated from nitrided TItanium-based Superelastic alloys Call name: Joint Research Projects Romania-France - IDROFR-2012 call
PN-II-ID-JRP-RO-FR-2012-0159 2012 -

Role in this project:

Coordinating institution: University of Bucharest

Project partners: University of Bucharest (RO); Institut National des Sciences Appliquees (FR)

Affiliation: University of Bucharest (RO)

Project website:

Abstract:

Organs like bone and vessels have a limited capacity for self-repair, and after injury or disease the regenerative power of adult tissue is often not sufficient, leading to non-functional scaring. Despite the vast development and clinical use experienced by the metal implants during the last decades these are far from optimal.

The objective of the present BIOMIMETIS project is to design and characterize new highly biocompatible titanium -based alloys for implantable devices in human body. In order to overcome the limited lifespan of the currently used biomedical alloys the proposed project envisages gas-nitriding treatments of recently developed Ni-free superelastic alloys (Gum-metal Ti-23Nb-0.7Ta-2Zr-O, Ti-24Nb-0.5O and Ti-24Nb-0.5N alloy compositions) which will harden the surface and improve further the biocompatibility.

The 3 partners involved in the frame of this project (2 academic laboratories and 1 industrial SME) composed of metallurgists, physico-chemists, biologists and mechanical engineers possess scientific competences and complementary skills in the domain of biomaterials in general and particularly in: (i) the design of superelastic Ti alloys and nitriding treatments, (ii) the physico-chemical and mechanical characterisations, (iii) the biocorrosion resistance evaluation, (iv) the biological validation of biomaterials and (v) the fabrication of biomedical devices.

Through a scientific and engineering program organised in 3 complementary tasks, the designed Ni-free nitrided superelastic titanium-based alloys will be deeply characterized to evaluate their functionalities for targeted biomedical applications. The fabrication of bone staple and endovascular stent prototypes by the industrial partner is envisaged as proof of concept in this proposal.

Thus, comparative microstructural and mechanical characterization of coated and un-coated superelastic titanium alloys, electrochemical characterization of the coated alloys’ surfaces before and after soaking in simulated body fluid (SBF) will be evaluated. Also, an evaluation of the fatigue behaviour of biomedical dispositives will be carried out. On the other hand, an important objective of our studies will be to evaluate the biocompatibility and to which extent the behavior of osteoprogenitor and mature endothelial and endothelial progenitor cells derived from umbilical cord blood is influenced by bulk material properties and surface characteristics. For the comparative purposes, the commercial nitinol, which is widely used in the biomedical sector, will be considered as a reference biomaterial. Furthermore, these studies will be preceded by cytotoxicity tests according to ISO 10993-5 standards. The osteoblast response will be assessed in terms of cellular survival, cell attachment, spreading and morphology and promotion of osteogenic differentiation. Endothelial cell behaviour will be approached by addresing cell adhesion representing a critical phenomenon for early endothelialization of the biomaterials, cell spreading and morphology, cell viability and proliferation, cell death, nitric oxide production and expression of endothelial differentiation markers. Because endothelial inflammation is a critical early event in vascular pathology, expression of inflammatory markers following cell-biomaterial contact will be assessed.

It is expected that the new Ti-based alloys will show excellent biocompatibility, low corrosion resistance in SBF and desirable mechanical properties combining the bulk superelastic property and the hard and wear resistant surface. These properties are particularly appreciated for various medical devices and, particularly, for targeted biomedical applications such as cardiovascular stents and orthopaedic bone staples. Furthermore, our expectations are that positive osteoblast response, a better endothelialization and a reduced inflammatory response will be obtained on the corresponding TiN-coated surfaces.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects