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Romania
Citizenship:
Romania
Ph.D. degree award:
2020
Mrs.
Aura - Elena
Ionescu
Scientific Researcher
Researcher
-
INSTITUTUL DE BIOCHIMIE
Researcher
4
years
Personal public profile link.
Curriculum Vitae (18/08/2023)
Expertise & keywords
Biochemistry
Projects
Publications & Patents
Entrepreneurship
Reviewer section
RECONSTRUCTION OF AN ANCESTOR OF RECEPTOR PROTEIN TYROSINE PHOSPHATASE CATALYTIC DOMAIN
Call name:
Exploratory Research Projects - PCE-2011 call
PN-II-ID-PCE-2011-3-0743
2011
-
2016
Role in this project:
Coordinating institution:
INSTITUTUL DE BIOCHIMIE AL ACADEMIEI ROMANE
Project partners:
INSTITUTUL DE BIOCHIMIE AL ACADEMIEI ROMANE (RO)
Affiliation:
INSTITUTUL DE BIOCHIMIE AL ACADEMIEI ROMANE (RO)
Project website:
http://www.biochim.ro/ib/projects/ancesptp/index.html
Abstract:
Receptor protein tyrosine phosphatases (RPTPs) have a pivotal role in regulating both normal and pathological cellular processes. Most RPTPs have two homologous intracellular domains but only the membrane proximal one is catalytically active. It has been broadly accepted that during evolution a hypothetical ancestral PTP domain duplicated leading to extant RPTPs. We propose in this project to reconstruct and characterize the catalytic domain of an ancestor of RPTPs. Initially, the ancestor gene will be synthesized then coded protein will be expressed in a prokaryotic system and purified to high purity. Similarly, several truncated and mutant forms will be obtained. The catalytic efficiency and substrate specificity under in vitro and in vivo conditions for all constructs will be evaluated and compared. It is expected that sequence of the ancestor PTP catalytic domain and its characteristics will consistently contribute to our understanding of the evolution of PTPs and the physiological function of the ancestor PTP. In addition, new insights into the role played by the molecular determinants of PTPs’ catalytic efficiency and substrate specificity will be generated. We expect that our results will help designing PTPs with defined substrate specificity.
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FILE DESCRIPTION
DOCUMENT
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects
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