BrainMap

Mr. Cristian Pirvu

PhD

Professor - UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Researcher | Teaching staff | Scientific reviewer | PhD supervisor

Web of Science ResearcherID: V-4619-2018

Expertise & keywords

An innovative sensing system for Non-Invasive Celiac Disease diagnosis Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-4405 2022 - 2024

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA NAŢIONALĂ DE ŞTIINŢĂ ŞI TEHNOLOGIE POLITEHNICA BUCUREŞTI (RO); INSTITUTUL NATIONAL PENTRU SANATATEA MAMEI SI COPILULUI "ALESSANDRESCU-RUSESCU" BUCURESTI (RO)

Affiliation:

Project website: http://inoceldetect.chimie.upb.ro

Abstract:

The scope of the present research project is to develop an innovative, simpler, fast, and more efficient system for the „no-biopsy approach” for Celiac disease (CD) diagnosis. The new system will be based on modified working electrodes with hybrid coatings containing conducting polymer nanostructures doped with quantum dots. We proposed to develop the new system in three stages. In stage one, modified working electrodes (WE) (glassy carbon) will be prepared. The change will consist of a hybrid-based coating with polypyrrole nanowires (PPyNWs) doped with graphene quantum dots (GQDs). Poly(amidoamine) (PAMAM) dendrimers will be used to create a surface suitable for biomolecule immobilization. Dendrimer can be grafted onto the surface of PPy through covalent bonding through electrochemical oxidation of amine groups of PAMAM resulting PPyNWs/GQDs/PAMAM. Adherent and uniformly distributed coatings on electrode surface, having lower resistance and with a large surface will be used in stage two. In the stage two, transglutaminase 2 (TG2) antigen will be immobilized on the modified electrode on PAMAM dendrimers. Electrochemical methods will be used to test the new system in the presence of known commercially available antibodies concentrations. We will use the same methods as in the previous studies: differential pulse voltammetry (DPV) and impedance spectroscopy (EIS). In stage three selected modified electrode will be used to monitor the reaction between the antigen and the anti-tissue transglutaminase 2 (anti-TG2) antibodies from the patient serum samples. Antigen-antibody complex formation produces changes in the surface conductivity, which is reflected in electrical signal. These changes are proportionally to the amount of Anti-Transglutaminase-2 antibody in the sample. The performance of the new system will be validated using currently available methods: TG2-IgA ELISA, the serum antiendomysial antibody (EMA) and the histologic analysis of the small bowel mucosa.

High performance phytosynthesized nanostructures as biosensor platforms for detecting pathogenic microorganism from food matrixe Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-0042 2022 - 2024

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA NAŢIONALĂ DE ŞTIINŢĂ ŞI TEHNOLOGIE POLITEHNICA BUCUREŞTI (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO)

Affiliation:

Project website: http://nanobiopat.chimie.upb.ro

Abstract:

Present project proposal “High performance phytosynthesized
nanostructures as biosensor platforms for detecting pathogenic
microorganism from food matrixes” is based on a modern and “green ”
method of obtaining mono and bi metallic nanostructures (mono and
bimetallic gold, silver copper nanoparticles) with the help of natural
extracts from cruciferous vegetables, in order to obtain sensing
platforms with high selectivity and specificity able to detect microbial
pathogens from food matrixes. The absolute novelty of the proposed
solutions and the foreseen results allows us to hope that potential
stakeholders (identified during the project) will be eager to take over
and implement these technologies, leading to social and economic
benefits, together with an important contribution to the increase of life
quality. The formulated concept of the product (related to the obtaining
of mono and bimetallic phytosinthesized nanostructures) and the
technology (the development of the sensing platform for detecting
pathogenic microorganism from food matrixes) will be the basis of the
project development. In the project will take place the analytical and
experimental characterisation of the product and technology that will
be developed (referring to the obtaining of innovative nanostructures -
TRL 3), the project being finished with the validation of the proposed
technologies at laboratory scale, through analyses performed via
third-parties laboratories (TRL 4). After the project implementation
period and protection of the technologies through patents, the
consortium aims at large scale prototypes and demonstration of the
functionality of the proposed solutions in real-life systems (TRL 6).

Electrochemical system for mercury ions detection Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-0730 2020 - 2022

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE -DEZVOLTARE PENTRU ECOLOGIE INDUSTRIALA - ECOIND (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://elsysmed.chimie.upb.ro

Abstract:

The scope of the research project entitled: Electrochemical system for mercury ions detection, acronym ELSYMED, is to obtain new devices based on screen printed electrodes (SPEs) modified with selective complexing polymeric film and coupled with a hand-held potentiostat for Hg(II) electroanalysis. Thus, the project corresponds to the aim of PN-III-CERC-CO-PED-2-2019 devoted to developing and testing demonstration models (functional experimental) for new systems with significant improvements, especially for metal ions detection from aqueous environmental samples.
The new system can fulfil essential tasks for on-site mercury ions detection such portability, low cost, rapid response and excellent sensitivity. ELSYMED project is based on our previous tested modified electrodes (laboratory tested) with 2,2'-(ethane-1,2-diylbis((2-(azulen-2-ylamino)-2-oxoethyl)azanediyl)) diacetic acid (L) which present selective behaviour towards Hg(II) ions. The ligand L will allow, by electropolymerization, to obtain selective SPs modified electrodes for Hg(II). Thus, by combining SPEs modified electrodes with portable potentiostat that runs with our unique electrochemical card (which contain the electroanalytical parameters) new electrochemical system for on site Hg(II) ions electroanalysis will be obtained.

Increasing the institutional bioeconomic research capacity for innovative exploitation of local plant resources to obtain horticultural products with high added value Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0332 2018 - 2021

Role in this project:

Coordinating institution: UNIVERSITATEA PITESTI

Project partners: UNIVERSITATEA PITESTI (RO); INSTITUTUL DE CERCETARE-DEZVOLTARE PENTRU POMICULTURA MARACINENI (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOTEHNOLOGII IN HORTICULTURA STEFANESTI-ARGES (RO); Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); STATIUNEA DE CERCETARE-DEZVOLTARE PENTRU POMICULTURA CONSTANTA (RO); UNIVERSITATEA DE MEDICINA SI FARMACIE CRAIOVA (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE IN SUDURA SI INCERCARI DE MATERIALE - ISIM TIMISOARA (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: https://www.upit.ro/ro/academia-reorganizata/facultatea-de-stiinte-educatie-fizica-si-informatica/departamente-la-nivelul-facultatii-de-stiinte-educatie-fizica-si-informatica/departamentul-stiinte-ale-naturii2/proiect-biohortinov

Abstract:

The complex project Increasing the institutional bioeconomic research capacity for innovative exploitation of local plant resources to obtain horticultural products with high added value comprises the following four projects:
Project 1."Complex electronic system with intelligent algorithms for data processing used for monitoring the developing conditions of water and biocenotic stresses, for warning about their presence and preventing them in horticulture"
Project 2."Multisensory quantification of water and biocenotic stress in horticulture through phytomonitoring and early warning in climate change conditions"
Project 3."Development of plant extracts and innovative phytosynthesized nanostructured mixtures with phytotherapeutical applications aimed at eliminating the biocenotic stress in horticultural crops"
Project 4."Innovative technologies for advanced processing of plant resources from pomiculture and viticulture"
The objectives of the project aim to develop the research capacity of the public organizations involved. Thus, upon the completion of the projects these organizations should meet the following outcome indicators: 1) employing new staff in research positions; 2)training the new and old employees through initial research training courses and specialist courses offered by each of the partners in their own field of competence (using the logistics of the learning resource centers); 3)obtaining new or significantly improved products/technologies/services; 4)structuring the offer for research and technological services and presenting it on the platform www.erris.gov.ro; 5)strengthening the capacity of the partner institutions through recovery strategies such as training programmes (research)/specialization programmes and visits (short-term); 6)offering and performing research services using the available research infrastructure; 7)creating a common programme for the learning resource centers, correlated with the institutional development plan of each partner

New modified electrodes for gluten detection from aliments Call name: P 1 - SP 1.1 - Proiecte de cercetare Postdoctorală
PN-III-P1-1.1-PD-2016-2185 2018 - 2020

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://tio2gluten.chimie.upb.ro

Abstract:

The project proposes the obtaining of modified electrodes with nanostructures and graphene oxide film, for detection of gluten in foods, including thermally processed foods. The project is intended to be a continuation of a research started during PhD studies, regarding nanostructures of titanium dioxide synthesis and characterization. In the proposed research, new modified electrodes will be obtained, to allowing a lower detection limit. Nanostructures of titanium dioxide are coated with a graphene oxide film. Graphene oxide - titanium dioxide combination recently atracted researchers attention because it provides an improved electron transport and has a high specific surface area. This could be an advantage, because the present proposed research is based on the use of an electrochemical detection method, differential pulse voltammetry. As the electron transfer will be faster, the signal will be better and will be able to provide a lower detection limit. This is a very important aspect for patients with celiac disease and for food industry. The proposed method could be used for mass production, providing them with a simple and fast detection instrument.
For thermally unprocessed food such as flour, detection is simpler because they do not have a large number of ingredients and gluten is not affected by termic or other modifications.
Thermally processed foods requires a prior extraction. This is because they have a large number of ingredients and gluten undergoes changes during processing. For these, a new method will be used involving a green deep eutectic solvent. In this the use of toxic organic solvents is eliminated.
To ensure the specificity of detection, on electrodes surface will be chemically modified with antigliadin antibodies. In this way is no longer required the use of enzymes or other modifiers, as is the case of classical, commercialy available Eliza tests.
The proposed method has the advantages of shorter time for modified electrodes prepa

Advanced green materials for photonics Call name: P 4 - Proiecte de Cercetare Exploratorie
PN-III-P4-ID-PCE-2016-0674 2017 - 2019

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://www.biomats.chimie.upb.ro

Abstract:

DNA photonics research is still at its very beginning. However, the up to date publications show huge potential of these biomaterials for applications in photonics and in electronics. DNA or DNA based complexes show very interesting photoelectric properties. The research on stability of active molecules in DNA-CTMA proves that the biopolymer matrix is much better as hosting material than synthetic polymers. Although the number of research laboratories, developing this field of research, as well as the number of practical applications on such materials, are still limited, it begins to change, as shown by the exponential increase of publications in last years. In this context ALL-BIO materials represent a challenge as up to day there are few researches and there are no devices based on such kind of materials. At this stage our goal is to face a new challenge in designing and promoting GREEN materials, a new concept based on integrating characteristics of smart, multifunctional advanced materials with the requirements of GREEN chemistry and sustainable development. It is expected that the outcomes of this project focused on research at the frontier of knowledge will open promising opportunities for increasing the competitiveness in the field of nanofabrication for biophotonics. This project defines its research strategy by introducing an original concept termed as “Bioactive mixture with unique biotronic properties”. In this respect the main objective of this project is Designing and obtaining INNOVATIVE GREEN materials with high applicative potential in Photonics and in electronics. This objective will be fulfilled by means of the following specific objectives:
• Building versatile methodologies for obtaining various natural, photosensitive extracts able to confer a high light responsivity to the functional biopolymers.
• Optimization and characterization of high optical quality films based on functionalized biopolymers with tailored properties for photonics.

Enforcing integrative exploratory research in life science & engineering on Zr and Zr alloy complex coatings. Mechanisms of coating elaboration and characterization. Call name: P 4 - Proiecte de Cercetare Exploratorie
PN-III-P4-ID-PCE-2016-0316 2017 - 2019

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://sciengcoat.rf.gd

Abstract:

The project is assigned to the thematic area of subdomain PE5.3 Surface Modification and Biomaterials PE5.7. The main objective is to enhance visibility of Romanian research at an international level. The project proposes surface bionic coatings at micro and nanolevel on Zr and Zr50%Ti alloy and will include the loading and release of melatonin, and the subdomain of Toxicology LS 7.5 will be involved.
The proposed surface modifications are:
1) modified surfaces based Zr/ZrO2 with 1D nanostructures as nanowires (via electrospinning) and nanotubes or nanochannels structures (via anodizing);
2) mixed oxide crystalline ZrO2, , amorphous TiO2 nanoarchitectures on Zr50%Ti alloy;
3) changing the phase crystallinity of ZrO2 and of mixed oxide ZrO2-TiO2 nanoarchitectures on Zr and Zr50%Ti, respectively, after thermal treatment;
4) elaboration of hybrid complex coatings introducing natural components as chitosan and/or hydroxyapatite on the modified surfaces of Zr and Zr50%Ti (with 1D nanoarchitecture);
5) introducing melatonin on/in the 1D nanoarhitecture modified surfaces of Zr and Zr50%Ti,with and without natural components addition. After Step I, that is the coatings elaboration, Step II will follow and it includes the characterization of the coatings.
The project infrastructure such as: SEM, XPS, electrochemical atomic force microscopy (EC-AFM), XRD, contact angle and charge at interface equipments will be upgraded with an integrated platform for in situ characterization of morphological and mechanical properties of biomaterials. Biological assays (identification of reactive oxigen species, cell viability, lactate dehydrogenase, reduced glutathione, alfa-actine, vinculin and fibronectin as adhesion molecules) will be part of a PhD thesis. The study will propose a patent, establishing action models for bio and non bio interactions of melatonin in a bionic coating. The dissemination at high level will include 16 ISI papers with a total influence score higher than 20.

Innovative surface design to extend the life and safety of dental and orthopedic implants Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-0910 2017 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://www.unibuc.ro/cercetare/promovarea-rezultatelor-cercetarii/proiecte/proiecte-cu-finantare-nationala/ped-97-2017/

Abstract:

NovSurf addresses a critical health issue, namely the increasing incidence of dental and orthopedic implant revisions due to implant associated infection and inflammation, by proposing a novel approach based on development of a surface topography of nanochannelar type on titanium implants by anodization, on which zinc oxide nanoparticles will be incorporated as antimicrobial agents. The newly designed surface is expected to reduce the susceptibility of titanium implants to microbial colonization, to enable the decontamination of implants if infection occurs and to support bone healing around the implant. In-depth characterization of physical properties, chemical composition and biological activity of modified titanium surface is envisaged. In vitro studies will evaluate its antimicrobial and anti-biofilm properties, its bioactivity, and inflammatory potential. Preliminary in vivo evaluation of the extent of bone regeneration after insertion in rats femur of surface modified titanium pins will provide efficacy data insuring the proof of concept by the end of the project. The project is built on two pillars of expertise afferent to the institutions involved, namely the strong background in the electrochemical deposition of TiO2 nanostructures on implant materials and their characterization at University Politehnica of Bucharest, and the excellent capabilities in assessment of microbial and cell-biomaterial interactions at University of Bucharest. The team gathers researchers with complementary expertise acting in a synergistic manner in order to solve an important health issue related to implant failure. If our expectation will be fulfilled, the findings may form a good foundation for further potential clinical validation of Ti-based implants with this innovative surface design as ready products for clinical applications.

Enhancement of bone defect repair by mussel-inspired TiO2-based nanostructures functionalized with bioactive phytomolecules Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1372 2017 - 2018

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://prof.unibuc.ro/prof-abil-anisoara-cimpean/ped-149-2017/

Abstract:

Despite the vast development and clinical use experienced by the metal implants, such as titanium (Ti), these are far from optimal. The use of bioactive coatings is one of the most promising strategies to promote appropriate surface-cell interactions at the implantation sites. In this context, the project scope is to immobilize bioactive phytomolecules of Herba Epimedii (EpFs) with osteoprotective effect on Ti surfaces nanostructured with TiO2 nanofibers and nanotubes via polydopamine (PDA) and nitrodopamine (NDA) films as intermediate layers, and to investigate how such surface functionalization would affect the osteoblast behaviour and inflammation responses of RAW264.7 cells. Finally, in vivo biological performance of the developed coatings will be assessed.
The two academic partners with complementary expertise participating in this project will be involved in the fulfillment of the following scientific objectives: Obtaining of nanostructured Ti/TiO2 surfaces, deposition of PDA- and NDA- based films and EpF-immobilization; In-depth characterization of the developed films deposited on Ti/TiO2; Evaluation of in vitro biological performance of the elaborated films; Obtaining of the demonstrator product (EpF functionalized PDA/NDA-coated Ti/TiO2 pins); Development of an in vivo experimental model for osseointegration of the demonstrator product. Thus, our starting point is a TRL 2 which represents a concept – DA is a bioadhesive. We have already verified this concept using a well-established procedure and now we take the advantages of this property for bonding EpFs and to test a related bioadhesive (NDA) from the same class. After implementation of the starting concept, this will be developed and validated from next points of view: physico-chemical surface properties and biological performance in order to prove the bioactivity and feasibility of the coating and functionalization technologies which correlates with a TRL 3.

Technologies and smart products for prevention and treatment of mastitis in productive ruminants, based on green chemistry of the composites for veterinary public health (GREENVET) Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0415 2014 - 2017

Role in this project: Project coordinator

Coordinating institution: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti

Project partners: Institutul National de Cercetare-Dezvoltare pentru Chimie si Petrochimie - ICECHIM Bucuresti (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU TEXTILE SI PIELARIE-I.N.C.D.T.P. BUCURESTI SUCURSALA BUCURESTI INSTITUTUL DE CERCETARE PIELARIE - INCALTAMINTE I.C.P.I. (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); ROMVAC COMPANY S.A. (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://www.icechim.ro/polimeri/proiecte/greenvet/

Abstract:

GREENVET project is a multidisciplinary answer for mastitis treatment in productive ruminants by integrating scientific research from outside discipline, inside the veterinary public health. The solution consists in innovative composites using green chemistry, supramolecular structures and intelligent materials using natural sources for one of the most expensive and health risky disease in livestock production. A final treatment will be developed as product, for internal market but with high export potential. The product consists in an integrated complex system with natural bioactive components (essential oils) as cure for sustaining or alternative to classical antibiotics, anti-inflammatories and cicatrizants with high impact on quality of life: problems of food supplies and quality, health and sustainable resources. GREENVET assumes a consortium (2 unique and large national research institutes, the largest technical university from Romania and the largest producer in Romania for veterinary products) with both experienced and young researchers. The management consist in the classical: planning, organizing and controlling in order to attain a minimum risk for project implementation (specific risk in multidisciplinary research), but is based on latest trend in the field -less graphs and more people- concept, by motivational approach and a proven background history of the collaboration between partners. GREENVET consortium experience in biomedical solutions is focused on the project working plan and provides outputs in terms of experimental models, prototype set, innovative technology, patent claim, dissemination in ISI journals and scientific events, documentation for product registration/or product notification. The budget breakdown and plan is well balanced to the resources to be committed in work complexity and less in time length of the task for people with different skills in multidisciplinary research.

Improvement of biomedical implant properties by surface nano-architecturing and antibacterial protection Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0855 2014 - 2017

Role in this project:

Coordinating institution: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU

Project partners: INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE CANTACUZINO (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); TEHNOMED IMPEX CO S.A. (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://www.icf.ro/pr_2014/PN-II-PT-PCCA-2013-4-0855/SursaDeFinantare.html

Abstract:

This project proposes the obtaining of a novel, advanced, biocompatible Ti-Ta-Ag bioalloy for orthopaedic and maxillofacial implants with complete innovative composition and properties. Two Research Institutes, one University and two SMEs will resolve in synergetic, multi and inter-disciplinary way the following main scientific objectives: a) elaboration of a novel, original ternary Ti-Ta-Ag bioalloy that contains only non-toxic and non-allergenic elements (Ti and Ta) and an antibacterial element (Ag), assuring increased corrosion resistance, very low ion release, high bioactivity and bacteriostatic activity to avoid adverse reactions in the human body; b) the functionalisation of the alloy surface by introduction of antibacterial nano-particles to increase the antibacterial properties; c) nano-architecturing of the alloy surface with nano /-tubes, /-channels, /-fibres which will stimulate the implant direct attachment and rapid bone healing; d) assessment of the alloy chemical composition and structural characteristics by high level techniques; e) determination of the alloy mechanical properties by static and dynamic tests; f) alloy long-term electrochemical behaviour and corrosion resistance in artificial human biofluids simulating the severe functional conditions of an implant, using modern electrochemical methods; g) monitoring of the quantity of ions (specially Ag+ ions) released into biofluids by high level techniques to know the release rate of Ag+ ions from the bulk alloy; h) establishment of the nano-architecture with the most optimal properties of the stability, roughness, porosity by world level techniques; i) determination of the bacteriostatic capacity of the new alloy before and after its surface functionalisation and nano-architecturing; j) evaluation of the alloy biocompatibility in as-cast, functionalised and nano-architectured state by in vitro and in vivo studies. The project technological objectives are: a) laboratory technology of the alloy synthesis by the modern melting method in vacuum levitation furnace with cold crucible; b) laboratory technology for the surface functionalisation with antibacterial nano-particles having suitable properties of the stability and antibacterial ability; c) laboratory technology for the surface nano-architecturing by the application of nano /-tubes, /-channels, /-fibres; e) two technologies for the orthopaedic and maxillofacial implants obtaining; d) fabrication of the orthopaedic and maxillofacial implants – demonstrator products. The original and innovative contributions of the project are: a) novel, biocompatible ternary Ti-Ta-Ag alloy; b) a new, complex functionalisation and nano-architecturing of the alloy surface; c) elaboration of the original mechanisms of electrochemical behaviour for the as-cast, functionalised and nano-architectured alloy in simulated physiological solutions; d) scientific novelties concerning the modelling of the physical processes at the interface between new bioalloy and simulated biofluids; e) monitoring of the bacteriostatic activity by the modern techniques; f) prime novelty determination of the bare and structured alloy biocompatibility by in vitro and in vivo tests; g) original data bank regarding physico-mechanical, electrochemical, anticorrosive, surface, biocompatible, antibacterial characteristics of the novel bioalloy. The obtained orthopaedic and maxillofacial implants will fulfill the multiple, complete functions and properties of a good implant, actually non-existing on market.

New nanostructured multifunctional coatings for orthopaedic implants Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0473 2014 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA BUCURESTI

Project partners: UNIVERSITATEA BUCURESTI (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU FIZICA LASERILOR, PLASMEI SI RADIATIEI - INFLPR RA (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); TEHNOMED IMPEX CO S.A. (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://www.unibuc.ro/n/cercetare/proiecte/PCCA_2532014.php

Abstract:

The materials used for internal fracture fixations and joint replacements are all currently made of metals. However, metallic implants suffer from two shortcomings, one being the poor interfacial bonding between the metallic surface and surrounding bone, and the other the higher rigidity of metallic implants than that of natural bone. Additionally, complications associated with implants due to bacterial-induced infections arise frequently. The implant anti-bacterial function set additional challenges for implant development besides the requirements for osteoblast fixation, mechanical, and biomechanical constraints, consequently in many cases almost incompatible demands have to be met. For osseointegration it is important to promote osteoblast fixation and to avoid bacterial adhesion at the same time. But, cells and bacteria respond differently to implant surface parameters and this gives an opportunity to look for an optimal solution. The present life expectancy of metallic implants is estimated at 10-15 years. Taking into account the ageing of the population, a second implantation becomes more and more often necessary. Surgical revision, however, can be twice as expensive as the primary operation and may lead to significant complications, including infection, deformity, pain, and loss of mobility. This research project is proposed to adress these issues.
A new metallic material (low elastic modulus titanium alloy) with two types of nanostructured coatings, titanium oxide nanotubes and carbon nanowalls, respectively, will be designed, developed and investigated for optimal implant fixation through innovative integration of material science, physics, chemistry and life science. Cerium oxide nanoparticles will be immobilized by electrodeposition to provide the coatings with antibacterial properties. This new beta-titanium alloy along with the new coatings will impart to orthopedic implants the excellent corrosion resistance, adequate strength, enhanced mechanical compatibility, and good bioactivity for promoting bone tissue regeneration and fixation of implants, and to prevent bacterial infections, offering an innovative solution to all of the issues faced by the present metallic implants.
Functionalized surfaces will be fully characterized for physical properties, chemical composition and biological activity. In vitro biological studies will include adhesion, proliferation and differentiation of osteoblasts, assessment of inflammatory cytokines, nitric oxide release and foreign body giant cell formation in macrophage cultures. In parallel Staphylococcus aureus and Escherichia coli adhesion and biofilm formation on experimental titanium surfaces will be analysed. Collation of all the data will be performed to select from the alternatives the optimal coating. As proof of concept, the fabrication of orthopaedic implant demonstrator is envisaged.
To achieve the research goals, nine work packages have been established, and a research team with all of the requisite expertise has been formed. We firmly believe that the synergism of this research team will allow us to successfully conduct this multidisciplinary project and push the frontier of the field of orthopedic biomaterials.
The results of this project will have favorable social and economic impacts on society because the quality of patient life using the new implants could be improved greatly. Moreover, a reduction in the need for revision surgery could translate into reduced health care costs. The broad impacts of this program will also be evident in our strong commitment to human resource development.

Improving oral and systemic health using dental works from modified alloys Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0869 2014 - 2017

Role in this project:

Coordinating institution: UNIV.DE MEDICINA SI FARMACIE - CAROL DAVILA

Project partners: UNIV.DE MEDICINA SI FARMACIE - CAROL DAVILA (RO); UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); INSTITUTUL DE CHIMIE FIZICA - ILIE MURGULESCU (RO); UNIVERSITATEA BUCURESTI (RO); R&D CONSULTANTA SI SERVICII S.R.L. (RO); DENTAL ART GROUP SRL (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://cercetare-umf.ro/proiecte_parteneriate

Abstract:

The project proposal entitled “Improving oral and systemic health using dental restorations from modified alloys” is addressed to the priority field 4 Health and has as goal the improvement of oral and systemic health of the population through implementing new methods of prevention and intervention at national level having capacity of international expansion. Having the central objective the improvement of the health level mainly through the offer of medical services of prevention, the project will contribute to the reduction of costs in the field of health. The offer is elaborated under the competent and representative direction of the University of Medicine and Pharmacy “Carol Davila”, the Faculty of Dental Medicine, another 2 Universities, 1 Institute of research of the Romanian Academy and 2 co-financing SMEs. This consortium is by definition inter- and pluri-disciplinary and exhibits synergy and complementarity due to the contribution of Medicine, Chemistry and Biology to the achievement of the central objective and it shall benefit of the un-financed support of an European partner (Universita’ del Piemonte Orientale “Amedeo Avogadro”) well known in the field of oral pathology, partner which shall participate to the activity of research and dissemination of results. This partner with experience and tradition will enhance the chances of the project both at level of the choice of the best methods of prevention and intervention in oral health and also at the level growing the visibility of the research. As derived objectives to achieve the central goal, the project takes into consideration the formation of target groups of patients according to the following criteria of selection: the detection of lichenoid reactions, of a metallic pigmentary lesions; the existence of dental alloys (CoCr, CoCrMo, etc. used at mass level) which favoured the release of ions and the absorption due to materials imperfections. The project will also form proof-lots including in the study a high number of patients comprising also patients who did not use dental alloys, fact which will allow the set out of the cause of the diseases and of the most appropriate treatments for all the studied cases. Both the scientific background and the expertise of the 6 partners as well as the richness of human and material resources represent a warranty that it is possible to overlap the knowledge about the creation of an improved alloy (CoCrZr or Nb) capable to reduce the potential for serious diseases such as Lichen Planus. The project analyses the sick tissue and the amount of ions released chemically and biologically and to compare the behaviour of the existing materials with that of our new alloy. The improvement of the dental alloy will aim at both the composition as well as the surface state at micro- and nano- level. The trial of these materials will be performed in simulating conditions respecting the corresponding regulations form the European space of operation including the norms of bio-ethics and bio-security and the informed agreement of the patient. The results of the project will be disseminated and exploited both at level of health services as well as at level of co-financing partners who will develop technology and innovating dental works from improved alloys at reduced costs, fact that emphasizes the co-relation between the thematic of the project and the strategy of development of the SMEs partners. Obtaining a patent and publishing 5 research works in international publications of high impact is a prognosis of result indicators that complete the process indicators. The benefits of implementing the project and its exploitation after ending will grow the quality of life through precocious detection of diseases and their treatment and also through the proposal of a new less dangerous and less toxic alloy. The co-financing SMEs will develop as well the commercialisation capacity subsequent to the production of materials with high market potential.

SENSORS FOR METALS BASED ON AZULENES MODIFIED ELECTRODES FOR WATER QUALITY MONITORING Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-2151 2014 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); CENTRUL DE CHIMIE ORGANICA AL ACADEMIEI ROMANE "C.D.NENITESCU" (RO); INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU OPTOELECTRONICA INOE 2000 INCD (RO); INSTITUTUL NATIONAL DE CERCETARE -DEZVOLTARE PENTRU ECOLOGIE INDUSTRIALA - ECOIND (RO); ANALIST SERVICE SRL (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: http://SEMEMA.inoe.ro

Abstract:

Electroanalytical chemistry can play a very important role in the protection of our environment. In particular, electrochemical sensors and detectors are very attractive for on-site monitoring of priority pollutants, as well as for addressing other environmental needs. Such devices satisfy many of the requirements for on-site environmental analysis. They are inherently sensitive and selective towards electroactive species, fast and accurate, compact, portable and inexpensive. Such capabilities have already made a significant impact on decentralized clinical analysis. Yet, despite their great potential for environmental monitoring, broad applications of electrochemical sensors for pollution control are still in their infancy.
The proposed research project entitled SENSORS FOR METALS BASED ON AZULENES MODIFIED ELECTRODES FOR WATER QUALITY MONITORING, acronyme SEMEMA can have a notable significance to the development of the knowledge in this area. Its theme regards the development domain of some electrochemical sensors based on modified electrodes with polyazulene complexing films, being a domain of major interested in the international research. The propose research regards the way to obtain some amperometric sensors sensitive to pollutants as the heavy metals, especially mercury, copper, lead and cadmium, but not limited to these species.
The project fits the global research in macrocyclic and macropolicyclic host systems, like crown eters and cryptands. From relevant molecules in supermolecular chemistry, capable to selectively link organic or inorganic species, either charged or not, there are some that can be considered elementary. They feature recognition properties on which, after functionalization, a thin layer of sensor can be obtained through electropolymerization. Typically, the transposition of properties from homogenous to a heterogeneous system has the following advantages: usage of a significantly smaller amount of ligand as compared with the same system in a homogenous state, the possibility of automating the analysis method in a technological flow (on-line system), miniaturization of the detection system.
This project fits the domain of materials, processes and innovative products (7), Technologies and high precision mechanical products and mechatronic systems (7.3), Equipment and high precision devices for testing and controlling the environmental parameters and primary food quality (7.3.10) and proposes research in obtaining new electrochemical sensors for metals in water. The project is based on research synergy of partners with multidisciplinary talents from two universities University Politehnica of Bucharest - coordinator CO, Ovidius University of Constanta – partner P2, three research and development institutes - the C.D. Nenitescu Centre for Organic Chemistry of the Romanian Academy – partner P1, the National R&D Institute for Optoelectronics – INOE 2000– partner P3, and Romanian National Research and Development Institute ECOIND– partner P4, as well as an economical agent interested in research in physical and chemical sciences SC ANALIST SERVICE SRL – partner P5.
The coordinator and its partners have a material infrastructure well suited for completing the project objectives and the necessary human resources (43 persons involved, out of which 30% are young). The described activities are matched with the human and material resources of partners, specifically: synthesis and characterization of organic compounds (P1), determination of electrochemical properties and film formation expertise (CO, P1), recognition properties in homogeneous phase (P2), experience in manufacturing and testing prototypes and patenting (P3), experience in testing and validation of new methods (P4), practical/current experience in testing water content using classical and advanced analytical methods (P5).

Novel battery based on ionic liquids and nanostructured anode Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-1561 2014 - 2017

Role in this project:

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); UNIVERSITATEA PITESTI (RO); GLOBAL RESEARCH S.R.L. (RO)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website: https://cssnt-upb.ro/pro/batiolnan/

Abstract:

Our project refers to the design and elaboration of a new type of high energy density electrochemical battery. This subject is strongly related to the research theme 2.1.4. “Promoting clean energy technologies, environmental protection measures and reduction of greenhouse gas” of the thematic area “Energy”. This relationship comes from several directions: electric cars, replacing those propelled by combustion engines, energy storage (coming mainly from non-constant “green” sources), increased efficiency of the batteries used as energy supplies of electronic instruments.
We propose a novel design of an innovative battery including new nanostructured anode materials, a new electrolyte, based on ionic liquids and new ions to replace Li, e.g., Al and Mg in the new substrate for anode.
An essential part of the challenge of the above battery is designing new nano-textured materials used in the negative electrodes of batteries. We shall try two types of new nanostructured anode materials: one based on titanium dioxide (TiO2 nanotubes and nanopowders) and intermetallic compound nanostructures like NiSn, CoSn and CoSb, in order to increase the specific area of the electrode and to improve the battery performance by a facile ion insertion. Using titanium dioxide (TiO2) as host anode material can be profitable because the cost of titanium oxide is low, the methods of obtaining controlled structures are well known and handy, and its structure is a welcoming host for cations. Intermetallic compounds, especially those of tin are very promising anode materials for Li batteries, and it seems that such structures are essential in the case of Mg.
The electrolyte proposed to be studied and used in this project is an ionic liquid synthesized by the combination of imidazolium based cation and TFSI anion, mixed with pyrrolidine (PY)-based ILs. In this way essential requirements for the electrolyte such as high conductivity, low viscosity, a well stable electrochemical window, and negative enough reductive potential value will be fulfilled.
New metals will be investigated to replace Li in our battery, because lithium is difficult to handle in normal conditions, relatively expensive and exhibits not high enough performance required by nowadays technologies. Magnesium and aluminum ions can be an alternative because of their higher valence which provides a distinct advantage in their capacity for energy density over the existing lithium-ion battery (1000 and 1060 Wh/kg respectively vs. 406 Wh/kg).
Several novelties are associated with the present project proposal: (i) the structure of materials intended to be produced using electrochemical processes such as TiO2 nanotubes and nanostructured intermetallic compounds for anodes; (ii) the insertion of magnesium and aluminum ions into the above mentioned anode materials; (iii) the use of more environmentally friendly ionic liquids as electrolytes in batteries; (iv) the design and implementation of a battery prototype. Additional novel aspects of this research will arise during the development of the experiments, such as the impact of metal impurities, the dendrite formation (mechanism, methods to inhibit their growth, influence on the charge/discharge cycles).
This application has a strong inter-, multi-, and trans- disciplinary character because it involves competence from many fields: electrochemistry regarded from both sides – chemistry and physics (electricity), mechanical and thermal investigations, etc.

NEW CONCEPTS AND STRATEGIES FOR THE DEVELOPMENT OF KNOWLEDGE OF NEW BIOCOMPATIBLE STRUCTURES IN BIOENGINEERING Call name: Complex Exploratory Research Projects - PCCE-2008 call
PN-II-ID-PCCE-2008-0248 2010 - 2013

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DIN BUCURESTI-DEPARTAMENTUL DE BIOCHMIE SI BIOLOGIE MOLECULARA

Project partners: UNIVERSITATEA DIN BUCURESTI-DEPARTAMENTUL DE BIOCHMIE SI BIOLOGIE MOLECULARA (RO); INSTITUTUL DE BIOLOGIE SI PATOLOGIE CELULARA NICOLAE SIMIONESCU-LABORATORUL DE CELULE STEM SI TERAPIE CELULARA (RO); UNIVERSITATEA POLITEHNICA BUCURESTI-FACULTATEA DE CHIMIE APLICATA SI STIINTA MATERIALELOR (RO); UNIVERSITATEA POLITEHNICA BUCURESTI-CENTRUL NATIONAL DE CONSULTANTA PENTRU PROTECTIA MEDIULUI (RO); INSTITUTUL DE CHIMIE FIZICA ILIE MURGULESCU (RO); UNIVERSITATEA BABES-BOLYAI CLUJ-NAPOCA, CENTRUL DE BIOMATERIALE, INSTITUTUL DE CERCETARI EXPERIMENTALE SI INTERDISCIPLINARE (RO); INSTITUTUL DE CERCETARE-DEZVOLTARE PENTRU CHIMIE SI PETROCHIMIE BUCURESTI (RO)

Affiliation: UNIVERSITATEA POLITEHNICA BUCURESTI-CENTRUL NATIONAL DE CONSULTANTA PENTRU PROTECTIA MEDIULUI (RO)

Project website: http://www.pcce248.weebly.com

Abstract:

IN TISSUE ENGINEERING (TE) THE COMBINED KNOWLEDGE FROM BIOLOGY AND ENGINEERING
IS DIRECTED TOWARDS THE POSSIBILITY TO RESTORE LOST OR DAMAGED TISSUE. THE
GENERAL AIM OF THIS PROJECT IS TO CONSOLIDATE A CROSS-DISCIPLINARY TEAM OF
COLLABORATING INVESTIGATORS TO CARRY OUT SOME CELL-SUPPORT CONSTRUCTS (CSC)
WITH POSSIBLE APPLICATIONS IN REGENERATION/REPAIR OF SOFT AND HARD TISSUES AND IT
DOES NOT ASSUME PRE-CLINICAL AND CLINICAL TRIALS. THIS APPLICATION CONTAINS THREE
SPECIFIC OBJECTIVES: 1- OBTAINMENT OF NEW SUPPORT 3-D STRUCTURES DESIGNED TO
CULTIVATE OSTEOBLASTS AND HUMAN MESENCHYMAL STEM CELLS (HMSC) TO OBTAIN CSCS
WITH CHARACTERIZED ARCHITECTURE AND MECHANICAL PROPERTIES, USEFUL IN BONE TISSUE
ENGINEERING; 2 - DEVELOPMENT OF REGENERATION STRATEGIES OF ADIPOSE TISSUE BY
IMPLANTATION OF PREADIPOCYTES IN 3-D HYDROGEL SCAFFOLDS, THAT MIMIC
EXTRACELLULAR MATRIX, DESTINED TO THE RECONSTRUCTION OF SOFT TISSUE DEFECTS
(SEVERE TRAUMAS, DEEP BURNS OR TUMOR RESECTIONS) AND 3 - STUDY OF THE EFFECTS OF 3-
D CULTURE AND GROWTH FACTORS ON THE CHONDROGENIC DIFFERENTIATION OF HMSC CELLS
TO OBTAIN SOME INVESTIGATION MODELS OF THEIR POTENTIAL IN CARTILAGE TISSUE
REGENERATION. THE PROJECT PRESENTS VIABILITY, INNOVATION, COMPLEXITY AND
INTERDISCIPLINARY EXCHANGE BECAUSE: 1 - IT IS A CONSORTIUM WHICH CONSISTS FROM
PARTNERS WITH COMPLEMENTALLY COMPETENCES WHO ENGAGE TO ACT AS A UNITY IN THE
FOLLOWING FIELDS: CELLULAR AND MOLECULAR BIOLOGY, CHEMISTRY AND PHYSICS OF
MATERIALS, ENGINEERING SCIENCES – IN ORDER TO GET ALL OBJECTIVES; 2 - THE PARTNERS
ARE STAFFS WITH A GOOD, STRONG REPUTATION ON THEIR FIELD, AND THEY HAVE THE
NECESSARY MANAGERIAL EXPERIENCE AS WELL AS THE HUMAN RESOURCES AND PERFORMING
EQUIPMENTS AND 3 - THE PARTNERSHIP WAS PARTIALLY CONSOLIDATED DURING PREVIOUS
COLLABORATIONS AND WHICH SUSTAIN IT. ACCOMPLISHMENT OF THE OBJECTIVES OF THIS
PROJECT CONSTITUTES A SCIENTIFIC CHALLENGE FOR ANY SCIENTIFIC TEAM AROUND THE
WORLD.

Technologies for fabrication and applications of nanoparticles and bi- and three-dimensional architectures of III-V semiconductor materials Call name: P 3 - SP 3.1 - Proiecte de mobilități, România-Moldova (bilaterale)
PN-III-P3-3.1-PM-RO-MD-2016-0242 2016 -

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA POLITEHNICA DIN BUCURESTI

Project partners: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO); Institutul de Inginerie Electronică şi Nanotehnologii ”D.Ghiţu” al AŞM (Academiei de Ştiinţe a Moldovei) (MD)

Affiliation: UNIVERSITATEA POLITEHNICA DIN BUCURESTI (RO)

Project website:

Abstract:

Propunerea de fata, care implica o stransa colaborare intre Universitatea „Politehnica” din Bucuresti – Centrul pentru Stiinta Suprafetei si NanoTehnologie (UPB-CSSNT) si Institutul de Inginerie Electronică şi Nanotehnologii ”D.Ghiţu” al AŞM (IIEN) din Chisinau, Republica Moldova, are ca obiectiv principal dezvoltarea unor noi tehnologii inovative pentru sinteza nanomaterialelor pe baza de compusi semiconductori ai grupelor III-V cu arhitecturi diferite (0D, 1D, 2D, 3D), inclusiv compozite tridimensionale ale acestora, avand aplicatii in domeniul catalitic, al senzorilor, in fotonica sau construirea dispozitivelor optoelectronice si biomedicale. Se au in vedere in special tehnologii electrochimice de preparare, care permit un foarte bun control al principalilor parametri de sinteza (e.g., potential aplicat), dar si un cost mai scazut comparativ cu tehnologiile clasice. Investigatiile experimentale se vor concentra pe (i) fabricarea matricelor nanoporoase si a nanomembranelor cu morfologie controlata pe baza de InP și GaP; (ii) prepararea nanofirelor de InP, GaP, InSb, GaN; (iii) depunerea nanoparticulelor metalice de Ag, Au, Pt, Pd cu dimensiuni si densitati controlate in matrici nanoporoase de InP, GaP și GaN; (iv) sinteza materialelor hibride multifuncţionale compozite aerografit/semiconductor III-V cu aplicatii in dispozitive optoelectronice si drept stimulatori ai proceselor biologice.

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects