BrainMap

EUGEN GHEORGHIU

- CENTRUL INTERNATIONAL DE BIODINAMICA

Researcher | Teaching staff | Scientific reviewer | Manager | PhD supervisor

Web of Science ResearcherID: not public

Expertise & keywords

Rapid Antibiotic Susceptibility Testing based on Drug Driven Bacterium Dynamics Call name: P 4 - Proiecte de cercetare exploratorie - PCE-2021
PN-III-P4-PCE-2021-1281 2022 - 2024

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/BacteriumDynAST/BacteriumDynAST.html

Abstract:

Non-invasive, label-free analysis of single cell dynamics, highly demanded by biomedical, biotechnological, biosafety and environmental applications, currently raises serious technological challenges. Founded on recent results and original research developments of the project leader and his team, BACTERIUMDynAST proposes label free time-lapse Electro-Optical microphysiology assessment of individual cell dynamics as a ground breaking concept in biosystems engineering and biohazard assessment. To advance and prove the concept, in the 33 months duration of the project, we address rapid Antimicrobial Susceptibility Testing (AST) challenges through an innovative design enabling physiomics data from hundreds of individual cells to be simultaneously monitored/assessed. BACTERIUMDynAST advanced control (capture and controlled focalization within the working distance of a high numerical aperture objective) of magnetically tagged target cells (e.g. bacteria), and AI assisted multiparametric data processing are seen as paramount in reaching the ultrafast phenotypic AST demands and the foreseen wide applicative impact.

BACTERIUMDynAST capability to provide cellular electro-optical images with high temporal and spatial resolution will facilitate creation of the next generation of (single)cell-based biosensing platforms likely to support breakthrough applications involving both natural cells (from pathogenic bacteria/fungi to circulating tumor ones) and synthetic cellular sentinels.

Accurate sensing platform for VOC detection in air quality control systems of smart cities Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-3090 2022 - 2024

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/AQVOC/AQVOC.html

Abstract:

Project AQVOC aims to improve the capabilities for air quality monitoring in smart city applications by development of a fast, sensitive and reliable device for detection of outdoor and indoor Volatile Organic Compounds (VOCs) based on the Magneto-Optical Surface Plasmon Resonance (MO-SPR). The validated demonstration model will be based on the SPR technique, magnetically augmented, towards a product that can be integrated in the market. The device will use available modular benchtop MOSPR equipment previously developed by our team combined with our portable SPR platform with imaging capabilities, patents pending. The sensitive area will consist in an array of spots of gas sensitive polymers and metallic oxides providing a specific “fingerprint” after interacting with individual VOCs. Data will be collected and analyzed using the Principal Component Analysis (PCA) approach to extract relevant data regarding the nature and quantity of the target VOC. The project starts from TRL2, and is expected to reach TRL 4 - the validation of the components and of the detection platform in the laboratory environment. Validating the platform will make an easier task for a future development towards integrating the whole setup into a complete model. After project completion, the AQVOC platform is intended to be further developed and checked to successively reach the next TRLs for being produced and reach the market. The project activities are sustained by a well-equipped infrastructure and a multidisciplinary team of researchers with expertise in all needed areas: modelling & data analysis, instrumentation and software development, thin film deposition and characterization, micro-fabrication.

Electrochemical assay for the rapid and sensitive detection of β-lactamase activity Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-2298 2022 - 2024

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/ELECTROLACTAM/electrolactam.pdf

Abstract:

Antimicrobial resistance is predicted to kill 2.4 million people in Europe, North America and Australia in the next 30 years and to cost up to 3.5 billion US dollars per year. The situation is especially worrying in Romania, where an average antimicrobial resistance prevalence rate of 42.6% of all infections, the highest in the EU, was reported in 2015. As a consequence, antimicrobial resistance has become a central issue at the top of the public health agenda of many countries around the world, including Romania. It was discovered that microbes can render β-lactam antibiotics ineffective by producing β-lactamases which are able to hydrolyze the β-lactam ring of such antibiotics. Therefore, there is a great interest in analytical methods which can detect the β-lactamase activity of bacteria while avoiding lengthy bacterial growth steps. In this context, the present project aims to develop a novel electrochemical assay that allows the sensitive (≤ 0.1 mU/mL) and rapid (≤ 30 min) detection of β-lactamase activity. The electrochemical assay will be suitable to measure the β-lactamase activity of bacteria after these are collected from clinical samples by immunomagnetic separation and before any bacterial growth step. Such an assay can contribute to reducing the time needed to treat patients with the appropriate antibiotic. However, during the present project we will approximate bacteria expressing β-lactamase with magnetic microspheres modified with β-lactamase (and a follow-up project is planned for taking the new assay to bacteria level). Four work packages will take the concept to a Technology Readiness Level of 3. Project feasibility is assured by the expertise of the project team, the relevant background knowledge produced by the project team, and the good infrastructure existing at the host institution. Dissemination and IP protection activities will also be carried out in order to increase the impact of the project.

Electrical field modulated aptasensors for proteins: sensitivity, selectivity and control of enzyme activity Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-2297 2021 - 2024

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/E-MAP/e-map.html

Abstract:

The project has at core the modulation of aptamers’ conformation by an electric field (EF) to control their immobilization and the binding of specific ligands. Two main objectives are set: (1) the pursuit of highly sensitive detection, demonstrated with an electrochemical aptasensor for β-2-microglobulin, the protein linked to dialysis–related amyloidosis and (2) a systematic investigation of the aptamer-lysozyme binding interface to reveal how the EF affects the binding selectivity and the enzymatic activity of the bound protein. The study focuses, respectively on: (i) the detection of soluble protein aggregates by coupled electrochemical-surface plasmon resonance (EC-SPR) and chemometrics, aimed at the pattern recognition of the aggregation in biopharmaceuticals; (ii) the controlled binding/release of lysozyme relevant for biocatalysis and the food industry where the enzyme is used as an antimicrobial agent. Significant investigative effort by EC-SPR, electrochemical-surface enhanced Raman spectroscopy, nanoimpact electrochemistry, binding studies with lysozyme mutants and chemometric analysis of the data will unravel correlations with the observed changes in the enzymatic activity in the EF. The project will strengthen collaborations with high expertise groups from Romania and the USA, open new research avenues, promote the scientific competitiveness of the team and develop the research infrastructure of the host institution, including by attracting young team members.

High resolution multiparametric dynamics at single cell level: virus detection by assessing cellular response to viral exposure Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-2432 2021 - 2023

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/DYNASCOPE/dynascope.html

Abstract:

Cellular-based sensing platforms face serious challenges before enabling full realization of the concept of “cell sentinels” against biological threats (in particular against viral infections, as stringently called into action given the SARS Covid 2 pandemics).

Grounded on the PL expertise and the resources of the International Centre of Biodynamics team, DynaScope advances a sensing platform for quantification of viral (particles) entry in model cells and characterisation of cellular dynamics associated with viral replication progress. The biosensing concept is based on label-free monitoring the dynamics of electrical, morphological, structural and metabolic properties of relevant intracellular structures upon viral exposure, at high spatial and temporal resolution. We achieve this through: (a) multiplexed/multiparametric assays, (b) use of bioengineered cells (c) analysis of time-lapse dynamical data. Via a broad biotechnology/ bioengineering effort we integrate phase (via SPR/QPI) and electrical impedance information within an AC electric field actuated plasmonic based EIS (P-EIS) microscopy setup. The envisaged platform will provide accurate, dynamic electro-optical (quantitative phase) maps of cellular changes corresponding to physiological states of both single cells (ACE2 HEK modified cells) and cell ensembles. The unique detection characteristics of the DynaScope are essential for applications in the fields of cell signalling, drug screening and hazard evaluation.

Multiplexed electro-plasmonic system with high sensitivity and specificity for analyte detection in real samples Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-1433 2021 - 2023

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/MEPS/meps.html

Abstract:

MEPS addresses the unmet needs of bioanalysis market for analytical means with increased sensitivity and robustness by developing a label-free, very sensitive and specific multisensing electro-plasmonic system, based on surface plasmon resonance (SPR) measurements, for simultaneous detection of multiple analytes in real samples. Therefore, a novel and more sensitive way to measure plasmonic based electrochemical impedance by angle-resolved SPR phase assays in a common-path interferometry scheme will be advanced. The setup will comprise a multiplexed phase SPR system to be developed using ICB proprietary technology and a high speed synchronized acquisition and processing system based on a CMOS sensor (~30000 fps), combined with a signal generator and microfluidics. A biomedical application will demonstrate system capabilities i.e. simultaneous and sensitive detection in undiluted serum of several cardiac biomarkers with key role in diagnosis, risk assessment, treatment, and supervision of cardiovascular diseases. As a low-cost and robust alternative to antibodies, aptamers will be used as biorecognition molecules. The sensor surface will be functionalized with a suitable layer for improved specific detection and reduced nonspecific response. The system will be validated by conventional methods. Due to its novelty and analytical relevance for label-free detection in real samples, the proposed system and aptasensors promise significant both scientific and socio-economic impact.

Core integration of novel functional, adaptive materials into a smart, highly sensitive analytical system for point of need environmental applications Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-M-SmartMatter 2020 - 2023

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); Consiglio Nazionale delle Ricerche - Istituto per la Tecnologia delle Membrane (IT); Centre National de la Recherche Scientifique (FR); Centro Analisi Biochimiche Sas (IT)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/SmartMatter/smartmatter.html

Abstract:

SmartMatter proposes novel adaptive functional materials featuring magneto-plasmonic properties and validated capability for boosting the detection sensitivity of greenhouse gases and low molecular weight compounds (LMWC). Three research institutes and two SMEs from Romania, Italy and France gather their expertise to achieve breakthrough adaptive 3D nano platforms - nanoparticle/dynameric conjugates – to enhance the analytic response of (magneto)-optical waveguides sensing chips and of the analyte specific interaction sites, identified as bottlenecks in reaching the analytic potential of portable, autonomous sensing devices.

The customized design of novel materials based on nanoparticle/dynameric conjugates envisaged by SmartMatter aims at achieving extreme sensitivities via innovative exploitation of the known high dependency of the analytic behavior of optical waveguide sensors (e.g. Surface Plasmon Resonance, SPR and Magneto Optic SPR, MOSPR) on the inner structure of the sensing chip. SmartMatter sensors become active, dynamic amplifiers of the target specific affine reactions that modulate their structure. This approach goes beyond the state of the art SPR type sensors, with fixed structure that passively transduce affinity reactions taking place above their surface.

Moreover, SmartMatter proposes a leap forward, warranted by the use of 3D dynameric reservoirs to amplify the sensitivity for LMWC, notoriously low in classical assays. Besides their role as framework for the optical waveguide (SPR & MOSPR), they will increase the absorption of small molecules in 3D multilayer dynamer-nanoparticles conjugates, to adaptively improve the accumulation of the analyte and thus further enhance the sensitivity of the assay for small molecules. In achieving this, the classical use of the affinity sensing platforms will be replaced by a constitutional strategy: the dynamic self-organization of components within systems will be controlled for exploiting their adaptive behavior toward the fittest binding structure to the affine compound, largest conformational change and accurate control of the interaction responses.

By combining the concept of interactive dynameric matrices/ networks with natural biocatalysts, and nanoparticles we provide a facile in situ strategy to adaptively improve the accumulation of the analyte and achieve bio-catalytic activity.

As a breakthrough, nanoparticles within the 3D nanoplatforms are set to play a double role: as framework for optical waveguide and as sites for interaction with the analytes therefore developments will be harmonised with tuning of the functionalization of nanoparticles with bioactive molecules such as enzymes, abzymes, aptamers able to interact with target gases and LMWC.

SmartMatter will achieve integration in a point of need smartphone based optical wave-guide analytical device, its testing and validation in a laboratory environment against greenhouse gases and LMWC for harvesting the full capabilities of the developed smart materials.

The project starts at TRL2 to successively reach TRL3 and TRL4, at its completion. An industrial advisory board will be established to facilitate further development after the end of the project and the transition of the technology to higher TRLs.

The system will be portable, have monitoring and interconnectivity capabilities thus, it is expected that in a subsequent step it will reinforce the achievement of the European strategic policy targets in terms of reduction of greenhouse gas emission by providing a flexible network of sentinel sensors. Wider impact is foreseeable for biomedicine, chemistry and energy fields where the new materials design warrants enhanced catalytic power, improved sensitivity and specificity and compatibility with portable formats. The forefront research merging nanosensors, nanomaterials and dynamers, the dissemination and training components of the project will ensure strengthened innovation excellence of the European research institutes both within and outside the project consortium, will contribute to improved competitiveness and strengthened industrial leadership (upon reaching market valorisation).

Portable biosensor coupled with a sampling drone for the in situ assessment of seawater toxicity Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-MARTERA-MOBILTOX-1 2021 - 2023

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); UMR CNRS 6144 GEPEA Equipe CBAC Université de Nantes (FR); Heliceo (FR)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/MOBILTOX/mobiltox.html

Abstract:

The preservation of the oceans is a major issue of the 21st century. In this context, the European Union is committed to protecting our seas and oceans, as indicated in the Marine Strategy Framework Directive. Despite this, there is today a significant lack of methods aimed at qualifying its environments, and in particular with regard to the impact of pollutants on marine ecosystems. The aim of MOBILTOX project is to contribute to this effort by providing a mobile platform for the in situ assessment of water toxicity as an early-warning system. The platform will combine two analysis complementary modules (biosensors) relying on biological indicators and a sampling drone. The first sensing module is based on whole microbial cells as indicator of overall toxicity (approach based on inhibition of respiratory activity- fluorescent sensors). It will be used to determine the toxicity level caused by the pollutants mixtures in the studied environments (harbours, coastal areas, etc.). This first analysis level will provide information about the overall quality of the environment (the aim is not to detect specific toxic compounds). The choice of the biological indicator is crucial, therefore the cells will be isolated from the targeted environments (coastal area, harbors, mouth of rivers such as the Danube (Romania) or the Loire (France) in order to ensure a good representativeness of the information collected by this first approach. The second module aims to detect specific groups of contaminants, i.e. inhibitors of photosynthesis such as herbicides and metals (main marine pollutants from run-off from agricultural land and industrial activities) via inhibition of photosystem II (PSII) immobilized on electrodes.

The sampling platform will be developped from an existing drone suitable for a deployement in these coastal environments (resistance to wind, waves,etc.) and equipped with a set of probes (pH, temperature,etc.). A sampling module will be integrated on the drone and the whole will be controllable remotely from the shore. The mobile platform prototype will be evaluated in harbors and coastal areas close to agricultural and river discharge zones in the Black Sea and the Atlantic and compared with standard chemical and ecotoxicity tests. A set of robust microbial strains isolated from marine environments and sensitive PSII complexes from marine phototrophic microorganisms are key project results facilitating further development of MOBILTOX in a commercial product.

Point-of-Care system for personalized diagnosis and assessment of treatment efficacy for allergies Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-PERMED-POC4Allergies 2020 - 2023

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/POC4Allergies/poc4allergies.html

Abstract:

Allergy is the most common chronic disease in Europe. Up to 20% of patients with allergies live with a severe debilitating form of their condition, struggling with the fear of a possible asthma attack, anaphylactic shock, or even death from an allergic reaction. The latest review issued by the GA2LEN Network of Excellence estimates that indirect costs due to inadequately treated allergy in the EU range between 55 - 151 billion euro per year (b€/y). If patients were treated appropriately, average savings of 142 b€/y could be realized. However, for effective treatments that cure the progression of the allergies in its pre-symptomatic stages, the access to a more differentiated diagnosis is critical.

A multidisciplinary team will advance the first low cost device for the simultaneous immuno diagnostic based on the detection of selected antibodies from sera sample. We will develop a point-of-care (POC) analytical platform for personalized diagnostic of allergies combining a novel portable Surface Plasmon Resonance (SPR) system and microfluidics. Aiming at hazelnut and peanut allergies we are tackling two of the most threatening and frequent food allergens. The diagnosis will be based on allergy related peptide epitopes recently identified at Fraunhofer IZI.

To avoid patient to patient variations in the serum composition, the antibodies of different Ig-classes subtypes in sera will be selectively pre-concentrated and prepurified by immunomagnetic separation. This will be a significant improvement over current allergy assays and in combination with epitopes, it enables a more precise and predictive personalized diagnosis. Device operation will be validated under laboratory conditions and the final configuration adapted for its use at the hospital level, matching the POC to patient environment. The envisaged platform will support improved clinical decisions and treatments also in other immunological disorders, fostering a more sustainable health-care system.

Innovative enzymatic coatings for electrochemical interfaces Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-M-ENZ4IFACES 2020 - 2023

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); INSTITUTUL DE BIOLOGIE (RO); Metrohm-DS S.L (ES); Rhodes University (ZA)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/ENZ4IFACES/enzi4ifaces.html

Abstract:

Stability is paramount in biocatalytic coatings. Maximising stability relies on using highly stable enzymes from extremophiles as catalysts. Enz4ifaces proposes high-performing, innovative enzyme coatings for sensor devices, answering the need for rapid and low-cost on-site analysis in the agro-food sector. Innovation is mainly directed at obtaining stable, highly active, and environmentally-friendly inks based on a novel aldehyde dehydrogenase from a hyperthermophilic archaeon. The coatings are applied on electrochemical interfaces by industrial manufacturing methods. The coated interfaces will be validated for (1) the detection of dithiocarbamate fungicides in grapes and tomatoes and (2) the detection of acetaldehyde in wines, relevant for the agro-food sector. The project starts at TRL 2 and reaches TRL 5, rallying partners from Romania, Spain, and South Africa aiming to increased competitiveness, scientific visibility and profit and enhanced Europe - South Africa collaboration.

Photosensitive nanotools for neuronal stimulation and rescue of degenerative blindness Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-EURONANOMED-nanoLight 2020 - 2023

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); IRCSS - Ospedale Policlinico San Martino (IT); Sobonne Universite (FR); Consejo Superior de Investigacion Cientificas (ES); Solems S.A. (FR); Nanoimmunotech, S.L. (ES)

Affiliation:

Project website: http://www.biodyn.ro/PROJECTS/nanoLight/nanoLight.pdf

Abstract:

One of the grand challenges of biomedical engineering is to develop methods for interfacing light stimuli with the nervous system. nanoLight will exploit the revolutionary concept of focalized neuronal stimulation without using microelectrodes or planar prosthetic devices and avoiding genetic manipulations. The goal is to compensate for nervous system pathologies in which neuronal degeneration has induced a specific loss of function. This will be obtained by developing novel photosensitive nanotools (PNTs) that, by virtue of their sub-micrometre size, can achieve a spatial discrimination that goes beyond single-cell resolution. PNTs can be delivered to the tissue with minimally invasive microinjections and be anchored to the membranes of target neurons to convert light stimuli into an electrical stimulation. The project will focus on three parallel strategies exploiting both organic and inorganic PNTs: (i) organic PNTs based on conjugated polymers (oPNTs); (ii) inorganic PNTs based on silicon photodiodes (iPNTs); and (iii) hybrid PNTs (hPNTs) based on both inorganic semiconductors (e.g., ZnO) and conjugated polymers. PNTs will be surface-functionalized to stably interact with the plasma membrane and mediate light-evoked activation of neuronal circuits. We have shown that 1st generation oPNTs and iPNTs are not endocytosed and preserve their extracellular location in cells and tissue for a long time. We obtained preliminary clues on the role of photogenerated charges in the photo-transduction process and in the resulting neuronal activation. In view of therapeutic applications to human diseases, nanoLight PNTs will target photoreceptor degeneration in Retinitis Pigmentosa (RP) and age‐related macular degeneration (AMD). Preliminary results indicate that subretinally injected PNTs are effective in rescuing light sensitivity and spatial discrimination in an animal model of RP. This will guide nanoLight biomedical engineering to develop new methods for interfacing optical stimuli with the nervous system for healing pathologies in which neuronal degeneration has induced a specific and irreversible loss of function.

Development of a Point of Need portable system for rapid assessment of high threat pathogens including bioterrorism agents Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-5155 2020 - 2022

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: https://www.biodyn.ro/PROJECTS/PATHSECURE/pathsecure.html

Abstract:

PathSecure aims to advance the ICB innovative technology for detection of specific agents by developing, testing and validating of a portable system, and of related working protocol allowing in field/point of need early recognition of relevant high threat pathogens including bioterrorism agents (bacteria, fungi and viruses), as well as appraisal of the viability of target bacteria and fungi. PathSecure addresses market and societal unmet needs related to security, public health and environment for effective sensing devices to support preparedness for bioterrorist attacks (use of pathogenic strains of microbes to cause disease or death and/or to harm the environment). Whereas cell detection based on electrical assays and magnetophoresis is already demonstrated, PathSecure proposes the entire assay, including the magnetic affinity capturing of the target germs be merely magnetically driven, without any moving parts, supporting faster analysis and automation.

The basic principles were formulated, the proof of concept demonstrated and supported by recent patents of PathSecure team. Hence, the project starts from TRL 2 and is set to successively reach TRL 3, and TRL 4.

The expertise of the project multidisciplinary team covers all needed areas from: modelling, data analysis and software development to instrumentation, microfabrication and microbiology that proved successful in establishing the proof of concept set-up and sensing protocol to be further developed within PathSecure.

Project activities are structured into 3 RTD workpackages, supported by one on management & dissemination. Briefly, WP1 will develop the system, WP2 will perfect the measuring protocols and WP3 will test and validate in laboratory environment PathSecure system capabilities to effectively assess target germs.

To attain the full technology impact, after project completion, the technology is intended to be further developed to successively reach all TRLs for being produced and reach the market.

Automated Analytical Platform for Rapid Antimicrobial Susceptibility Testing Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-4932 2020 - 2022

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: https://www.biodyn.ro/PROJECTS/AutoAST/autoast.html

Abstract:

The scope of the proposed project is the development and validation of an Automated Analytical Platform for Rapid Antimicrobial Susceptibility Testing (

Multiplexed point-of-care device for cardiovascular biomarkers Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-4934 2020 - 2022

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: https://www.biodyn.ro/PROJECTS/CARDIOSENSE/cardiosense.html

Abstract:

Cardiovascular diseases (CVDs) are considered a major threat to global health. CVD biomarkers from blood, are important diagnostic indicators for CVD: cardiac Troponin I (cTnI) and T (cTnT), B-type natriuretic peptide (BNP), C-reactive protein (CRP) and myoglobin are the most relevant biomarkers for angina, myocardial infarction and acute heart failure. These biomarkers are currently analyzed in specialized laboratories by expensive and time-consuming procedures (assay duration spanning several hours). These procedures are unsuitable for rapid detection of biomarkers in point of need settings, as a response to life threatening situations where early detection of blood biomarkers is vital, such as in the risk of myocardial infarction. Therefore, there is a growing demand for portable, rapid and low cost biosensing devices for the detection of CVDs. As a response to this challenge, CARDIOSENSE aims to develop a novel point-of-care (POC) multiplexed diagnostic system for CVD biomarkers detection in undiluted plasma, enabling early, fast and affordable diagnosis sensing. CARDIOSENSE is based on a ground-breaking proprietary surface plasmon resonance (SPR) technology recently developed by ICB to be coupled to a smartphone. BNP, CRP, cTnT, cTnI and myoglobin will be simultaneously detected by sensitive angle resolved SPR measurements in very low volumes of undiluted plasma (~10 µl). A low-cost portable centrifugation system will allow rapid plasma separation from the blood cells. As a low-cost and robust alternative to antibodies, the system will use aptamers as biorecognition molecules. The novel system will allow biomarker levels detection in minutes, will be cost effective, compatible with mass-production, and could be tailored to other biomarkers with minimal technology change. After testing and validation in clinical trials, the POC device is envisaged to be used for rapid diagnosis of CVD in point of needs in hospitals (emergency departments).

Rapid, quantitative identification of microorganisms in a lab-chip assay Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-5185 2020 - 2022

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: https://www.biodyn.ro/PROJECTS/BactoID/bactoid.html

Abstract:

The development of methods for the rapid identification of microorganisms is a major step towards efficient biotechnology products, food and water safety control and accelerated clinical diagnosis of infectious diseases. Despite significant efforts in the development of new analytic tools for pathogenic cells assays (aimed for identification, enumeration and rapid phenotyping), cell cultivation on selective solid media remains the gold standard in microbiological analysis. BactoID proposes the development, testing and validation of an experimental prototype of a novel multiparametric electro-optic platform with microarrays allowing analysis of chromogenic spots as an effective tool for rapid, sensitive and specific identification of microorganisms, translatable in bench top and portable formats. BactoID aims to shrink the time lag of colony identification on solid media, typical in classical microbiological analyses by integrating affinity magneto capture and magnetic guidance at microscale, with arrays of selective and differential chromogenic media in a lab-chip format compatible with multiparametric, high resolution electro-optic assays.

BactoID project harnesses the available knowhow and technological resources within the International Centre of Biodynamics (ICB) and proposes to substantially advance the ICB innovative research concerning a) detection and quantitation of target analytes, b) the lab-on-chip approaches for multimodal modification and evaluation at microscale, in synergy with other validated platforms within ICB. BactoID platform will allow rapid, sensitive identification of pathogenic bacteria, validated in laboratory on selected microorganisms (chosen, without restricting the applicability to other domains, for enhanced relevance for the overarching fields of Biotechnology and Medicine) upon successful completion of the project.

Portable biosensor based device for mapping the risk of grape infection by Botrytis cinerea in the vineyard Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-MANUNET-III-WINBIOTOOL-2 2020 - 2022

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); Enkoa System S.L (ES); BODEGAS DE LOS HEREDEROS DEL MARQUES DE RISCAL, S.L. (ES); THE WHITEAM CONSULTING (ES)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/WINBIOTOOL/winbiotool.html

Abstract:

The biggest challenge faced by the wine industry is the grape infection by Botrytis cinerea, affecting 1520%

of the harvest worldwide. WINBIOTOOL aims to provide a much needed tool for wine producers to answer this

challenge, in the form of an integrated detection platform, combined with mapping and decision software. This

tool allows the in-field, early detection of fungal attack by Botrytis cinerea and screening laccase activity in

grapes and throughout wine production, recommending the best decision for minimizing the impact of the fungal attack. The instrument includes electrochemical biosensors, GPS sensors and web application and combines KETs (Key Emerging Technologies) and ICT (Information and Communication Technologies) to map the fungus spread in the vineyard. Starting at TRL2, the project will advance to TRL4, delivering an instrument tested at winery level as main outcome of high added value. Carried out by 4 SMEs/Industrial partners and 2 Public Research Centers, WINBIOTOOL will provide a product that will facilitate obtaining high quality/yields of wine production and will contribute to the European companies’ competitiveness in the global wine industry.

LIGHT DRIVEN DYNAMICS FOR CELL BASED SENSING: A NEW TWIST FOR OPTOGENETICS Call name: P 4 - Proiecte de Cercetare Exploratorie
PN-III-P4-ID-PCE-2016-0762 2017 - 2019

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/PROJECTS/DRIVESENS/drivesens.pdf

Abstract:

Cell-based biosensors employ living cells and sensors or transducers to detect changes of the intra- and extra-cellular microenvironment and cellular physiological parameters, as indicators of the interaction with external stimuli. Nevertheless, the long response times, addressability to cell population, cell and parameter specific assays relative nonspecificity and high biological variability hindering the applicability of current cell based biosensors rise the challenge of radically new approaches in cell based biosensing effective also in the context of weak or delayed cellular effects, e.g of low concentration toxic compounds. To respond to this challenge, DriveSens proposes selective induction of an exogenously driven rhythm and evaluation of the dynamics of cell recovery under control conditions (as reference) and in relation to target stimuli/analytes towards a cell based biosensing platform with inner referencing, multiparametric capabilities and rapid, label free response with applicability to a wide class of analytes, including (low concentration) toxic compounds. Optogenetic control of nonelectrogenic cells is integrated into a noninvasive electro-optical analytical platform enabling evaluation of the dynamics of cellular response induced by target exogenous stimuli at both single cell and cell population levels.

In an interdisciplinary approach connecting optogenetics to electro-plasmonics and microfluidics, DriveSens will reveal the “zoom in” capabilities of the proposed method to harness cellular adaptation mechanisms to a well controlled exogenous stimulus, for amplifying cell response to weak or long range bioactive stimuli.

DriveSens will significantly impact basic research e.g. via dynamic, multiparametric insights into signal transduction pathways of the cells involved in normal and pathological interactions, as well as applicative (biosensing) areas concerning cell signalling, bio/nano-materials testing, drug screening and toxicology evaluation.

Cell Biosensors for Detection of Chemical and Biological Threats_NATO-SPS 985042 & UEFISCDI-PN3-329/42/2017 Call name:
NATO-SPS 985042 & UEFISCDI-PN3-329/42/2017 2016 - 2019

Role in this project: Partner team leader

Coordinating institution: University of Bologna

Project partners: University of Bologna (); CENTRUL INTERNATIONAL DE BIODINAMICA ()

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA ()

Project website: http://www.cell-biosensors.org/

Abstract:

A rapid response to a chemical or biological terrorist attack, as well as to an accidental release of toxic environmental pollutants, requires the ability to monitor and detect chemical or biological agents, so that an early warning can be raised, potential health risks defined, and proper countermeasures are employed. We propose a battery of bioengineered live cell biosensors "tailored" to respond to different agents by the generation of measurable and quantitative dose-dependent signals. A selection of such cell biosensors will be integrated into a portable robust device and used as “living sentinels” for on field terrorism and pollution surveillance.

Mobilitate cercetător EUGEN GHEORGHIU Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetatori, 2019
PN-III-P1-1.1-MC-2019-2487 2019 - 2019

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website:

Abstract:

Graphene-based optoelectrochemical sensor for the simultaneous monitoring of the electrical and chemical activity of single cells Call name: P 3 - SP 3.2 - Proiecte ERA.NET
FLAG-ERA-Grafitivity 2016 - 2019

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/PROJECTS/Graphtivity/Graphtivity%20Project.pdf

Abstract:

Improved understanding of molecular principles of neuronal communication allows new insights into neurodegenerative diseases and possibly to new therapeutic targets. However, there is a lack of tools able to monitor simultaneously electrical and chemicals signals of single cells.

Therefore, we propose the development of a novel graphene-enhanced sensor allowing simultaneous monitoring of these two signals with single cell resolution both in cell cultures and organotypic tissue slices. The sensor will be based on surface plasmon resonance imaging (SPRi) and its ability to provide excellent spatial resolution also to electrochemical measurements. More importantly, by integrating graphene both the sensitivity of SPR detection and the current densities of the electrochemical measurement will be enhanced with concomitantly improved

biocompatibility. In addition to generating new knowledge about the interplay of electrical and chemical signals of living cells, the development of the anticipated sensors will be an important step towards novel prostheses based on the bidirectional communication with living cells.

The core of the sensor will be a “cell chip” carrying disk microelectrodes, to which cells adhere, surrounded by cell-free ring microelectrodes. Once cells have adhered to the disk microelectrodes, the ring microelectrodes (bare or modified with enzymes) are polarized to a potential that allows oxidation or reduction of signaling molecules secreted by the cells. Subsequently, high-resolution SPR images of the “cell chip” are recorded at high frame rates (~10000 fps) while a physical or chemical stimulus is applied to the cells. SPR images of cell-covered

disk microelectrodes are modulated by changes in the extracellular field potential of the cells (which enables us to monitor e.g. the propagation of action potentials). SPR images of the ring microelectrodes will be altered by changes in local current densities invoked by variations in the local concentrations of signaling molecules and will be used to observe chemical signals from the cells.

Neuronal cells change their extracellular field potential within the low millivolts range and release only tiny amounts of signaling molecules. Therefore, the sensitivity of SPRi has to be improved in order to be able to record electrical and chemical signals from cells simultaneously. Graphene has already been proved to enhance the sensitivity of both SPR and electrochemical detection. Hence, graphene and its derivatives will be applied for signal amplification.

Our optoelectrochemical approach to measure extracellular field potentials with sub-micrometer resolution will excel voltage sensitive dyes and electrically interrogated microelectrode arrays. In addition, it will provide unprecedented spatial resolution and interference elimination to the electrochemical monitoring of chemical signals from cells.

Advancement of a Portable System for Fast and Sensitive Detection of Pathogenic Cells Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1041 2017 - 2018

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/PROJECTS/FindPathogen/findpathogen.html

Abstract:

FindPathogen aims to advance the ICB innovative technology for target cell detection by developing, testing and validating of a functional, portable, standalone kit, and of related working protocols allowing for fast, sensitive and specific in field/point of care detection of relevant pathogenic cells, including strains that acquired resistance to antibiotics, as well as appraisal of the alive or dead status of target cells. Therefore, FindPathogen addresses market and societal unmet needs for effective bioanalysis kits to provide assurance along the entire food supply chain, from farm to retailer’s shelf, as well as for health applications (e.g. rapid and accurate microbial diagnostic).

The basic principles were formulated, and the proof of concept on cell detection using periodic magnetic actuation is already demonstrated, published and supported by recent patents of FindPathogen team. Hence, the project starts from TRL 2 and is set to subsequently reach TRL 3 (laboratory tests performed to measure parameters of interest and comparison to analytical predictions for critical subsystems), and TRL 4 (validation in laboratory environment).

The expertise of the project multidisciplinary team covers all needed areas from: modelling, data analysis and software development to instrumentation, microfabrication and microbiology that proved successful in establishing the proof of concept set-up and sensing protocol to be further developed within FindPathogen.

Project activities are structured into 3 RTD work-packages, supported by one on management & dissemination. Briefly, WP1 will develop the analysis kit, WP2 will perfect the measuring protocols and WP3 will test and validate in laboratory environment the capabilities of the kit and sensing methods.

To attain the full technology impact, after project completion, the kit & measuring protocols are intended to be further developed and checked to successively reach all TRLs for being produced and reach the market.

Electro-plasmonic system with improved sensitivity for analyte detection Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1279 2017 - 2018

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/PROJECTS/EPSIS/epsis.html

Abstract:

As a response to the needs of bioanalysis market for biosensors and analytical means with increased sensitivity and robustness, the EPSIS project will develop a highly sensitive electro-plasmonic system for affine detection of target analytes. The system will employ simultaneous surface plasmon resonance (SPR) and faradaic electrochemical impedance spectroscopy (EIS) detection of analytes solely by plasmonic measurements (EC-SPR), taking advantage of SPR signal dependence on both surface charge density and refractive index variation near the interface and in bulk. In a simplified, unitary set-up, the advanced system will provide both SPR and EIS responses.

While the basic principles and technology supporting SPR/EC-SPR and related biosensors were already formulated and sustained by a series of publications, the project starts from TRL 2 and is set to subsequently reach TRL 3 (laboratory tests performed to measure parameters of interest and comparison to analytical predictions for critical subsystems), and TRL 4 (validation in laboratory environment).

Existent infrastructure and expertise of the project multidisciplinary team covers all needed areas: modelling, data analysis, software and instrumentation development, thin film deposition, surface chemistry and fluidics.

Intermediary results of the EPSIS project, namely the effect of gold layer thickness in conjunction with the applied electric field to EC-SPR signal and a sensitive biosensor in terms of specific and non-specific binding will have a significant impact on biosensors field. High throughput analysis will also benefit from EPSIS, since EC-SPR can be used in imaging mode, therefore providing spatial resolution.

Sensitive Platform for Dynamic Assessment of cellular effects of target endo- and exo- genous compunds: case study Amyloidβ Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1137 2017 - 2018

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/PROJECTS/SENSECELL/sensecell.html

Abstract:

Despite significant advances in diagnosis and monitoring of neurodegenerative diseases, fundamental controversies related to the pathogenic mechanisms, the structure of the causative agents and the active cellular targets still persist, emphasizing the need for cellular platforms as “breadboards” for development and testing of new therapeutic approaches.

In line with Programme scope, and responding to unmet needs in bio-medical and pharmaceutical assays related to neurodegenerative diseases, SensCell project aims at developing, testing and validating in a laboratory environment a platform for multiparametric evaluation of cellular effects induced by endo-/exo-genous compounds, elucidation of interaction mechanisms and assessment of the effectiveness of new therapeutic interventions.

SensCell advances the ICB innovative research concerning a) label free sensing of amyloid ﬁbrils effect on living cells b) evaluation of dimers in oligomeric and aggregated mixtures and c) lab-on-chip approaches for multimodal alteration and evaluation of cell microenvironment. SensCell proposes an integrated analytical platform to evaluate the efficiency of AD treatment and development of in vitro models able to recapitulate key aspects of complex neuro-degenerative diseases.

Without restricting the general applicability, SensCell structure enables the access to cellular models with complex architecture integrated with complementary physiological screening modalities relevant for both basic research and drug development and opens up future exploitation pathways in theranostics, in expediting the assessment of drug effectiveness and safety in diverse pathologies.

Thus, the project innovatively addresses the current knowledge frontiers and the limitations in access to effective treatments for Alzheimer’s disease and preclinical testing, tackling with Public Priority Area: Health, in particular, the research in neurodegenerative diseases, Pharmacology and quantitative systemic toxicology.

Electro-Plasmonics for the analysis of the dynamics of cellular processes and biomolecular interactions - BioScope Call name: Complex Exploratory Research Projects - PCCE-2011 call
PN-II-ID-PCCE-2011-2-0075 2012 - 2016

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); SC INSTITUTUL DE OPTOELECTRONICA SA (RO); CENTRUL INTERNATIONAL DE BIODINAMICA (RO); Universitatea din Bucuresti (RO); CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/php/biodyn.php?act=Projects&idm=5&idsm=11&locale=en

Abstract:

Whereas experiments on processes at single cell level are highly demanded by biomedical industry they currently raise serious technical challenges. Therefore, BioScope project advances the State of the Art by proposing creation of the Electro-Plasmonic Analysis System (EPAS), an innovative engineered system integrating advanced optics and electronics that will reveal the dynamics of electrical and morphological properties of relevant intracellular structures and biointerfaces with an unprecedented temporal (1 ms) and spatial (200 nm) resolution.

A multidisciplinary consortium will develop EPAS by harnessing Surface Plasmon Resonance, SPR, Magnetism (to increase SPR and thus overall sensitivity), Optogenetics (to control cell properties through light for calibration purposes), Modelling (to reveal details of how cells in electric field affect SPR), Bioinformatics (for the analysis of data) and Biochemistry (to validate/substantiate the results).

Novel analytic capabilities of EPAS to perform fast, noninvasively and label free, thorough in situ measurements for gaining new insights into cellular processes (such as changes in adherence, conformation, and organelle distribution, swelling / shrinking, exo- and endocytosis, neurotransmitter release, electrical spiking, etc.) at single cell level will be explored.

BioScope results will facilitate creation of the next generation of (single)cell-based analytical platforms likely to support breakthrough applications spanning from quality control of food and aquatic media to bio-medical ones coping with analysis of interaction mechanisms between selected cells and various stimuli (including those fostering new pharmacological products), cell assays as alternatives to animal (preclinical) testing, or last but not least, supporting cutting-edge cell-based “Disease in a Dish” approaches.

Sensitive quantitation of target microorganisms using dual electro plasmonic analysis and magnetic actuation Call name: Postdoctoral Research Projects - PD-2012 call
PN-II-RU-PD-2012-3-0467 2013 - 2015

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/PROJECTS/Contract%20No.%2082-30.04.2013/Contract%20No.%2082-30.04.2013.html

Abstract:

The aim of this project is the development of a method for fast detection of microorganisms in liquid samples with increased sensitivity by advancing dual SPR-EIS analysis in conjunction with periodic (magnetic) actuation. The concept is envisaged to overcome the limits of current methods regarding response time, sensitivity and robustness by capturing the target cells with modified magnetic beads (MB) and using specially designed sensing chips.

To this purpose the following objectives are envisaged:

1. Controlled capture of the analyte close to the sensitive area of the sensor chip.

2. Develop a sensitive analytical platform based on dual SPR & EIS assays and on periodic magnetic actuation for detection of microorganisms (case study on Escherichia coli O157:H7); establish the detection limits and reproducibility

3. Validation of results (using a luminescence method).

Project results are expected to have a wide applicative impact by providing effective means to improve the existing approaches for detection and quantitation of microorganisms with applications in quality control of environment and food, as well as in bio-medicine.

The project activities will also support strengthening of Project Leader scientific carrier by acquiring knowhow on advanced modelling and data analysis, microfabrication, as well as by mastering SPR and EIS techniques.

project title Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetatori
PN-III-P1-1.1-MC-2018-3013 2018 -

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website:

Abstract:

Unconventional Optical Imaging Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetatori
PN-III-P1-1.1-MC-2018-0051 2018 -

Role in this project: Project coordinator

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website:

Abstract:

Bioengineered cell biosensors for detection of chemical and biological threats Call name: NATO "Science for Peace and Security"
SPS 985042 2016 -

Role in this project:

Coordinating institution: Centrul International de Biodinamica

Project partners: Centrul International de Biodinamica (RO)

Affiliation:

Project website:

Abstract:

A rapid response to a chemical or biological terrorist attack, as well as to an accidental release of toxic environmental pollutants, requires the ability to monitor and detect chemical or biological agents, so that an early warning can be raised, potential health risks defined, and proper countermeasures are employed. We propose a battery of bioengineered live cell biosensors 'tailored'to respond to different agents by the generation of measurable and quantitative dose-depedent signals. A selection of such cell biosensors will be integrated into a portable robust device and used as 'living sentinels' for on field terrorism and pollution surveillance.

project title Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetători cu experiență din diaspora
PN-III-P1-1.1-MCD-2017-0159 2017 -

Role in this project: Project coordinator

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website:

Abstract:

SPIE-Photonics West -Quantitative Phase Imaging IV Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetatori
PN-III-P1-1.1-MC-2017-2066 2017 -

Role in this project: Project coordinator

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website:

Abstract:

project title Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetatori cu experienta din diaspora
PN-III-P1-1.1-MCD-2016-0013 2016 -

Role in this project: Project coordinator

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website:

Abstract:

FILE DESCRIPTION	DOCUMENT
List of research grants as project coordinator
List of research grants as partner team leader
List of research grants as project coordinator or partner team leader
Significant R&D projects for enterprises, as project manager
R&D activities in enterprises
Peer-review activity for international programs/projects