BrainMap

Lorant Janosi

Ph.D.

Senior Researcher (Scientific Researcher I) - INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M

Researcher | Scientific reviewer

ResearcherID: http://www.researcherid.com/rid/C-1074-2012

Curriculum Vitae (09/01/2019)

Expertise & keywords

Design of New Lipid-Modified Peptides to Destabilize Ras Nanoclusters - A Novel Therapeutic Approach for Targeting Oncogenic Ras Proteins Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-2418 2015 - 2017

Role in this project: Project coordinator

Coordinating institution: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M

Project partners: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M (RO)

Affiliation: INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M (RO)

Project website: http://www.itim-cj.ro/PNCDI/ru118/

Abstract:

Ras proteins mediate a wide variety of signal transduction pathways that regulate cell growth,proliferation and differentiation.These proteins are small GTPases acting as binary switches between the GDP-bound “off” and the GTP-bound “on” states.Oncogenic mutations of Ras renders them constitutively active and are associated with ~15% of all human cancers and up to 90% in specific tumors.Current strategies for developing drugs targeting Ras mutants had little success.Experiments and computer simulations alike showed that membrane-bound Ras proteins form nonoverlapping dynamic nano-sized subdomains (nanoclusters) in an activation state-/isoform-dependent manner.Nanoclusters are protein-lipid assemblies serving as exclusive sites for effector recruiting and signal activation.The main objective of this application is the development of a new approach on finding potential drugs against oncogene Ras.The ground-breaking nature of this approach is that it exploits the dynamic nature of Ras nanoclusters and the key role of their stability in signal transduction.We will computationally determine Ras self-association binding sites and their binding strengths.The results will be used to design new lipid-modified peptides(LMPs) which,due to their designed features,would disrupt Ras nanoclusters.Since Ras nanoclusters are highly dynamic in nature,the peptide-Ras interaction should disrupt nanocluster’s stability and hence the signal output.

Ion sensing and separation through modified cyclic peptides, cyclodextrins and protein pores Call name: Complex Exploratory Research Projects - PCCE-2011 call
PN-II-ID-PCCE-2011-2-0027 2012 - 2016

Role in this project: Key expert

Coordinating institution: “Alexandru Ioan Cuza” University

Project partners: “Alexandru Ioan Cuza” University (RO); National Research and Development Institute of Isotopic and Molecular Technologies (RO); “Babes-Bolyai” University (RO); “Horia Hulubei” National Institute for Physics and Nuclear Engineering (RO); “Carol Davila” University of Medicine and Pharmacy (RO)

Affiliation: National Research and Development Institute of Isotopic and Molecular Technologies (RO)

Project website: http://science.research.uaic.ro/biosens/

Abstract:

Development of nanostructures capable of detecting and separating individual molecules and ions has become an important field of research. Particularly, protein-based nanostructures are attractive due to their ability for tunable molecular recognition and ease of chemical modification, which are extremely important factors on various applications. In this project, self-assembly functionalization will be approached, aimed at providing an efficient design for molecular recognition, ion sensing and separation, through new host-guest chemical methodologies, bio-nanofabrication and physicochemical manipulations methods. New crown ether type macrocycles, functionalized cyclodextrins and cyclic peptides will be engineered to work as specific molecular adaptors for the -hemolysin protein, giving rise to hybrid molecular superstructures possessing ion sensing and selectivity properties. The size and functionality of the macrocycles are targeted to ensure the anchorage in the pores and the selectivity of specific host-guest complexation processes. A surface detector array device suitable for use with a biosensor is envisioned, through ink printing nanotechnologies. The device architecture will be formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. Custom designed nanoscale bilayers containing selected receptors through cyclodextrins derivatives and macrocyclic peptides, self-assembled on different micro-nano arrays surfaces (polymers, Au or Si) will be fabricated. Further engineering of such functionalized nanomaterials based on molecular recognition and host-guest methodologies, in conjunction with flexible and mechanically robust enough substrate platforms, have the great potential for applications such as separation of nanoparticles, sensors, drug delivery, removal of heavy metals from aqueous solutions and chiral separation.

Association of Aquaporin Transmembrane Channels and Influence of their Oligomerization State on the Transport Properties Call name: EC-FP7
FP7-94759-254470 2019 -

Role in this project: Project coordinator

Coordinating institution: UNIVERSITA DEGLI STUDI DI CAGLIARI

Project partners: UNIVERSITA DEGLI STUDI DI CAGLIARI (IT)

Affiliation: UNIVERSITA DEGLI STUDI DI CAGLIARI (IT)

Project website:

Abstract:

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