BrainMap

Mrs. Loredana Florina Leopold

Dr.

Professor - UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Researcher | Teaching staff | PhD supervisor

Web of Science ResearcherID: AAA-5812-2022

Curriculum Vitae (15/06/2025)

Expertise & keywords

Innovative solid-state fermentation system for enhancing the nutraceutical content of agrifood by-products Call name: P 4 - Proiecte de cercetare exploratorie - PCE-2021
PN-III-P4-PCE-2021-0750 2022 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation:

Project website: https://pce0750.wixsite.com/website

Abstract:

Nutraceuticals (NLs) are biomolecules (carbohydrate derivatives, polyphenolics and fatty acids/structural lipids, carotenoids) that have health-promoting, disease-preventing, or medicinal properties. NLs are found in small amounts in dietary sources, thus it is necessary to develop procedures to increase their concentration and extractability. The agri-food by-products could be appropriate for using them as raw materials in the production of these industrially relevant bioactive compounds under solid-state fermentation (SSF) conditions. The aim of the present project is to develop, for the first time in the field, improved SSF processes using encapsulated fungal spores (individual and mixed-cultures), with different functions/ properties/ biosynthesis mechanisms to enrich simultaneously some abundant agro-industrial by-products (brewer's spent grain and grape pomaces) with valuable hydrophilic (polyphenolics) and lipophilic (polyunsaturated fatty acids and β-carotene) biomolecules.

Nanoplastic toxicity on vegetable crops - assessment of accumulation and effects at the morphological and phytochemical level Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente - TE-2021
PN-III-P1-1.1-TE-2021-1585 2022 - 2024

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation:

Project website: https://sites.google.com/usamvcluj.ro/plastimpactresearch/home

Abstract:

Microplastic (MP) and nanoplastic (NP) enter agro-eco-systems either as primary micromaterials (e.g. textile fibers, coatings, adhesives), or indirectly, generated by the breakdown of larger plastic debris. Although the fates of plastics, especially of MPs in marine environments are being increasingly well studied, little is known about the behavior of NPs in terrestrial environments. Due to its smaller size, NP presents a higher internalization potential, being thus linked to a higher toxicity. Therefore, we aim to study morphological and molecular changes induced by NP exposure, by studying the uptake, bioaccumulation, biodistribution and toxicity of NP on in vitro plant cultures of Stevia rebaudiana. Bioaccumulation and biodistribution will be assessed by conventional fluorescence imaging and hyperspectral fluorescence imaging for labelled polystyrene (PS), polyethylene (PE) and polypropylene (PP) NPs of 30 and 100 nm size. The plants will be grown by exposing to following NP concentrations 10, 50, 100 and 250 mg/l. Also, Raman imaging will be employed for monitoring unlabelled NPs in vegetal tissue. To assess the phytotoxicity induced by NPs the following compounds responsible for stress due to toxicity will be quantified: chlorophylls, carotenoids, glycosides, soluble vitamins, volatile organic compounds and malondialdehyde (MDA). Thus, the results of this study will contribute to a better understanding of the risks of exposure to NPs.

Development of novel nanocarriers for enhanced physico-chemical properties, bioactivities and stability of essential oils Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-1847 2021 - 2023

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation:

Project website: https://soniasocaci.wixsite.com/nanoseo

Abstract:

Essential oils (EOs) are aromatic volatile oily liquids extracted from different parts of plants, known to possess various bioactivities, including antioxidant, antimicrobial and antiproliferative activities. Despite their numerous proved health benefits, EOs are very sensitive to environmental factors (light, heat, moisture, oxygen) when used as such, having a high volatility, strong odour and poor water solubility. These drawbacks limit their application as active compounds in food, pharmaceuticals or cosmetics. To overcome these shortcomings, the nano-sized delivery systems are considered as one of the most promising technologies. Nanocarriers can be used for entrapment of EOs to uphold their original characteristics during processing and to improve their physico-chemical and functional properties. Thus, the present proposal is focused on the development and comprehensive characterization of three innovative nanoformulations: (i) EOs nanoemulsions, (ii) EOs loaded in chitosan nanoparticles and (iii) EOs-metallic nanoparticles colloidal dispersions. The developed nanoformulations will provide the protection of the EOs bioactive compounds, enhancement of their stability, bioavailability, controlled release and hence in their effectiveness as antioxidants, antimicrobials or antiproliferative agents.

Demonstration models for meat analogues and confectionery products based on extractive products from conventional and alternative oilseeds and their blends - Development and Lab validation Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-5346 2020 - 2022

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://vladmuresan3.wixsite.com/fagaceae

Abstract:

More and more people combat the extensive farming of livestocks for milk and meat production, soy derived and highly processed ingredients consumption, which nowadays are the global pillars of protein supplying, and from various ethical, cultural, religious and ideological hindrances refuse to consume these types of products. Consequently, global demand for new protein sources has focused mainly on different sources oilseeds. The aim of this project is to develop, characterize and validate in a laboratory environment, demonstration models for meat analogues and confectionery products based on extractive products from conventional and alternative oilseeds and their blends. This novel approach to the processing and utilization of whole sunflower and Fagaceae seeds (acorn and beech seeds) will be accomplished by: O.1 Formulation of different food compositions for laboratory experimental demonstrators – thereof technology and advanced characterization (phytochemicals, structure, colloidal stability, rheology), respectively O.2 Validation at laboratory level the optimal developed experimental demonstrators by assessing their process-ability, sensory, microbial alteration, phytochemicals degradation and oxidative stability. The transition to more reliable food models – similar as those from current project proposal demonstration models – will give way to the functional, nutritionally complex and sustainable prospected resources and raw products, to be part of every person daily diet.

Functional collaboration model between public research organisations and the private sector for high-level scientific and technological services in the bio economy field Call name: P 1 - SP 1.2 - Proiecte complexe realizate in consorții CDI
PN-III-P1-1.2-PCCDI-2017-0056 2018 - 2021

Role in this project: Partner team leader

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO); STATIUNEA DE CERCETARE -DEZVOLTARE AGRICOLA TURDA (RO); UNIVERSITATEA BABES BOLYAI (RO); UNIVERSITATEA TEHNICA DIN CLUJ - NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://sweetconomy.com

Abstract:

The aim of the project is to develop a functional collaboration model between public research organizations and the economic environment in order to provide high-level scientific and technological services in the field of bioeconomics. The objectives of the project are: O1: Creation of a consortium between public research organizations (USAMV, SCDA TURDA, UBB, UTCN) and the economic environment; O2: Increasing institutional capacity through scientific and technological services, through human resources expertise to improve institutional performance; O3: Increasing the institutional capacity to capitalize on the results of the research, by the functioning of the technological transfer component to the economic environment, by publishing and invention patents; O4: Reduction of 30% sugar addition in fermented foods and obtaining a consortium of atomized lactic bacteria. These general objectives are based on the following scientific objectives: Os1: Cultivation of 3 soybean varieties in 3 systems of soil cultivation and characterization of soybean chemical profile; Os2: Evaluation of the toxicological impact of nanoparticles in soybean and the development of a biosensor to identify titanium, zinc and gold nanoparticles accumulated in the plant and grain; Os3: Selection of lactic bacteria and optimization of fermentative processes on soybean and wheat substrate, by mathematical modeling, for the production of polyols to reduce sugar addition in food; Os4: Microinjection of the consortium of lactic bacteria by atomization, envelope aseptic packaging for industrialization; Os5: Educational program on nutrition with soy products, based in the community.The main impact of this proposal is reflected by the creation of new jobs for researchers, sustainable and after the completion of the project, by expertise the human resources and by training the young researchers in modern techniques for the re-launch of SCDA Turda.

New integrated approach for food waste valorization Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2016-0973 2018 - 2020

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://soniasocaci.wixsite.com/intvalwaste

Abstract:

Large amounts of wastes are generated annually by the food industry, their efficient management and valorisation representing one of the main objectives of EU actions against food waste and towards sustainable development. Citrus is one of the most important fruit crop in the world. About 50–60 % w/w of the processed citrus fruits becomes waste which if not processed further, can give rise to serious environmental pollution. CW contains a wide range of bioactive compounds, such as essential oils, carotenoids, fibre, flavonoids, which have many applications in food, cosmetic and pharmaceutical industry. Despite the existing studies on exploitation of bioactive molecules from food wastes, there is still a struggle and need in finding the most efficient methods that could also be economically viable. In this context, the project aims the development of a procedure for the sequential recovery of bioactive compounds from CW, towards achieving the “zero-waste” desiderate. The envisaged bioactive compounds to be recovered from CW, are essential oils (EO), antioxidant compounds (phenolics, flavonoids) and dietary fibres. The performance of the proposed procedure will be assessed (i.e. yield, possibility to be scaled-up) as well as the functionality and bioactivities of recovered biomolecules (i.e. antioxidant capacity, antimicrobial activity, emulsifying properties) using modern analytical techniques. By means of mathematical modelling and statistical analysis of data, the best working parameters will be selected for the design of integrated multi-step system for CW bioactive compounds recovery and valorization.

Smart polyelectrolyte multilayer capsules for controlled release of bioactive compounds Call name: P 1 - SP 1.1 - Proiecte de cercetare pentru stimularea tinerelor echipe independente
PN-III-P1-1.1-TE-2016-1907 2018 - 2020

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://www.smartpems.com/

Abstract:

Unceasing efforts are currently made by researchers worldwide to improve drug delivery systems. Despite the nanobiotechnology advances in functionalising nano- and micro-particles particles for delivery agents and contrast therapies, there is still a great need for nanoparticles and microparticles that can specifically bind, stabilise and deliver therapeutic agents inside human cells and tissues. Here we propose desiging an optically addresable, controlled release delivery system with low based on polyelectrolyte multilayer capsules. The system should facilitate controlled, targeted release of the cargo upon exposure to laser light and. The multifunctional system is based on carotenoids and gold nanoparticles encapsulated in a polyelectrolyte multilayer matrix. A laser mediated procedure will be used to facilitate the carotenoid content release. Carotenoids will be used as model system for the bioactive compound. The gold nanoparticles are used in connection to their plasmonic properties. Under laser irradiation they are expected to transform light into heat and to facilitate the release of the carotenoids. Such a controlled release system would have the clear advantages of improving the delivery of bioactive compounds, with great potential of applications in the field of nanomedicine.

SusTainable explOitation of toMATO proCessing industrY by-produCts Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-MANUNET II -TOMATOCYCLe (1) 2017 - 2020

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://tomatocycle.weebly.com

Abstract:

The objective of this project is the holistic approach for the utilization of tomato processing byproducts.

These wastes could be considered valuable by-products if the appropriate technical means, in

terms of economic impact, is used and if the value of the subsequent products is expected to exceed the cost of reprocessing. The cultivation and processing of tomatoes is the largest agricultural business in Southern European countries. Every year millions of tons of tomatoes are used and large amounts of tomato byproducts are treated as waste. These waste quantities from tomato processing industry constitute an important ecological and also financial problem. The current applying practices, regarding the treatment of tomato processing by-products, include mainly the usage as animal feed and / or disposal. However, being a rich source of natural bioactive compounds, tomato waste will be utilizing to produce high value-added products for food, cosmetics, or pharmaceutical applications along with new food formulations and improved animal feed and organic fertilizers.

Anthocyanins pure extracts and their involvement in biological studies: potential bioactive ingredients in nutraceuticals Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1002 2017 - 2018

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: http://anthoextract.com/

Abstract:

The proposed project brings a new approach to a very common problem in Biotechnology today.

Anthocyanins, natural plants pigments found in very significant amount in edible berries and many vegetables, showed to have high antioxidant activity. Having antioxidant potential, anthocyanins have been highly investigated regarding their health benefits for humans. Unfortunately it is not clear how exactly anthocyanins act at cell level, but their properties seem to be closely related to their antioxidant activity. Even if many researchers are investigating their potential health benefits, almost all bioassays on anthocyanin-rich commodities have been performed using crude anthocyanin extracts from fruits and vegetables, without eliminating other potentially bioactive impurities. These impurities consist mostly of other phenolic compounds and are very likely to have biological effects and may greatly influence biological assays. Based on the fact that anthocyanins are responsible for all potential health benefits the proposed project aimed to develop a low cost method that will allow the obtaining of high purity (>90%) anthocyanin pure extract. Other types of compounds like phenolic acids and flavonoids will be individually separated and recovered from the crude extract. Starting from these pure extracts, comparative tests on cell lines will be performed in order to prove that the benefits of anthocyanin rich materials are due to the anthocyanins themselves or due to other compounds present in those extracts, or even due to the interaction between anthocyanins and other compounds in the crude extracts. The project will offer a complete overview of how much is the real contribution of anthocyanins to the health benefits from anthocyanin-rich materials. Moreover based on the laboratory results, the low cost purification method will be a starting point for industry application offering basic ingredients for next generation of nutraceutical products.

Efficient utilization of biodiesel crude glycerol in the production of L-lactic acid Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2016-1237 2017 - 2018

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO); UNIVERSITATEA TEHNICA DIN CLUJ - NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://biofitped.weebly.com

Abstract:

Biodiesel represents an alternative for the substitution of fossil fuels but generates a huge amount of crude glycerol which causing the environmental problems regarding the management of this waste. A solution to reduce the negative environmental problems and the cost of biodiesel is to use crude glycerol as a carbon source for Rhizopus oryzae growth in order to produce L-lactic acid. The project aims are to develop and validate the improvement production of L-lactic acid using crude glycerol as a substrate, immobilized spores, and inhibitors for alcohol dehydrogenase of R. oryzae in discontinuous and semicontinuous fermentation processes in order to make the process more efficient.Our preliminary results, generated (Vodnar et al., 2013) sustainable opportunities for L (+) lactic acid production via pelletized R. oryzae NRRL 395 fermentation on biodiesel crude glycerol.The objectives are well - correlated with the outcome of the projects and are as follows:O1: Immobilization of R.oryzae spores in polyvinyl alcohol cryogel O2: ADH inhibited R.oryzae capacity to produce L-lactic acid from crude glycerol during discontinuous fermentation (flask and small bioreactor) O3: ADH inhibited R.oryzae capacity to produce L-lactic acid from crude glycerol during semicontinuous fermentation (flask and small bioreactor) O4: Optimization of the L-lactic acid production conditions using a fractional order mathematical model. The BioFit system proposed starts from TRL 3 where the previous experiments demonstrated the concept functionality through significant results, and will move to TRL 4, more precise to the validation of the improvements added to the existing system using mathematic model. The use of crude glycerol from biodiesel production present a great opportunity for lactic acid production in order to defray the production cost of biodiesel and to promote the biodiesel industrialization on a large scale.

Innovative structuring technologies for cold pressed vegetable oils: "Omega(ω)-rina" – spreadable food product rich in polyunsaturated lipids and plant antioxidants Call name: P 2 - SP 2.1 - Cecuri de inovare
PN-III-P2-2.1-CI-2017-0037 2017 - 2017

Role in this project: Key expert

Coordinating institution: TAF PRESOIL SRL

Project partners: TAF PRESOIL SRL (RO); UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: https://vladmuresan3.wixsite.com/go-omega

Abstract:

The objectives proposed in this project meet the needs identified at beneficiary company (www.lunasolai.ro), by (1) evaluating the oxidative stability of cold pressed vegetable oils and (2) the development of innovative technologies for structuring cold pressed vegetable oils. To achieve the first objective it is aimed to optimize the storage conditions (storage temperature and time), including an inert atmosphere packaging and adding spices extracts to enhance stability while maintaining products bioactives. Sensory analysis, major oil quality parameters (acidity, iodine value), primary and secondary oxidation indices (hydroperoxides, conjugated triene, para-anisidine, TBARS) will be monitored periodically for all cold-pressed oils of the beneficiary (sunflower, pumpkin, flax, walnuts, canola and hemp oils). Additionally, for walnut oil (the most susceptible to oxidative degradation), for samples stored under controlled conditions, volatiles markers of oxidation will be identified and quantified by GC-MS, the infrared fingerprint while calibration models coupled with chemometrics will be developed. Regarding the second objective, to control and better understand the phenomena of gelling cold-pressed "LunaSolai" oils, phase diagrams, microstructure and techno-functional properties will be studied by infrared spectroscopy, microscopy, thermal and advanced rheological characterization. Moreover, the incorporation of spices or essential oils and extracts in the developed oleogels, will emphasize the innovative character of this service. The intellectual property for the new spreadable fat product rich in polyunsaturated fatty acids and plant antioxidants will be protected by filing a patent.

Anthocyanins liposomes systems and their involvement on molecular signaling of skin diseases Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-0944 2015 - 2017

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: http://antholipoenc.com

Abstract:

Proiectul propus aduce o nouă abordare pentru o problemă de actualitate în domeniul Biotehnologiilor. Antocianii sunt pigmenţi naturali, care au atras interesul nu doar datorită culorilor din fructele şi legume ci şi datorită faptului ca sunt molecule biologic active cu beneficii pentru sănătate. Cele mai bogate surse de antociani sunt fructele de pădure, precum afinele, soc, aronia, zmeură, legumele şi unele cereale. Antocianii prezintă o serie de proprietăţi benefice, dintre care cea mai importantă constă în efectul lor antioxidant.

Fiind o macromoleculă hidrosolubilă, antocianii sunt instabili și foarte sensibili la degradare. Pentru valorificarea potenţialului benefic al acestora, sunt necesare sisteme de livrare controlată şi care le protejează proprietățile. Un bun candidat pentru acesta sunt lipozomii. Incapsularea antocianilor ȋn lipozomi ca sistem de livrare şi protecţie a acestora are două provocări: încapsularea antocianilor în lipozomi nu este bine înțeleasă și documentată. O altă provocare va fi de a evalua potențialul lor antiproliferativ pe celule de melanom după ȋncapsulare. Încapsularea antocianilor în lipozomi, cu o rată ridicată de încapsulare şi păstrarea proprietăților antocianilor după încapsulare va fi de mare folos pentru comunitatea științifică și poate deschide noi posibilitati de utilizare a antocianilor ȋncapsulaţi ȋn lipozomi pentru tratamente alternative în bolile dermatologice.

Microencapsulated plasmonic nanoparticles for controlled release of bioactive molecules Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-2211 2015 - 2017

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: http://MicroBioNAu.com/

Abstract:

The use of functionalised particles for targeted therapies and imaging, and understanding their effect on cell functions are among the current challenges in nanobiotechnology and nanomedicine. Despite recent advances in functionalising particles for targeted delivery agents and contrast therapies, there is still a great need for nano- and micro-particles that can specifically bind, stabilise and deliver therapeutic agents inside human cells and tissues. The present project addresses two different structures with sizes in the nanoscale and microscale range, namely plasmonic gold nanoparticles and polymeric microcapsules. Basically, the aim of the project is to encapsulate the plasmonic nanoparticles in a biocompatible polymeric matrix with the final goal of reaching controlled and effective release of the loading from the capsules. The research work will be focused on the manufacturing of polymer encapsulated plasmonic nanoparticles, testing ways to open the capsules and release their content, and in vitro testing of biocompatibility of nanoparticle loaded polymeric capsules, their cellular uptake, cellular localisation, distribution inside the cells, and intracellular release. The research activities will make use of modern techniques: high resolution electron microscopy (TEM/SEM), confocal microscopy, molecular spectroscopy techniques (Raman, SERS, FTIR), and performing in vitro experiments on cell cultures.

BIOCONVERSION OF AGRO-FOOD WASTES INTO VALUE-ADDED FUNGAL METABOLITES Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-1255 2015 - 2017

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: http://evadulf.wix.com/pn-ii-ru-te-15

Abstract:

The worldwide agricultural and food industries produce annually large amounts of wastes, which cause serious environmental problems by direct disposal of these by-products to soil or in a landfill. In recent years, the researchers are making significant efforts to reduce the volume of these wastes by finding alternative uses. Because of the sugars- rich composition, which due to their organic nature are easily assimilated by the microorganisms, the agro-food wastes could be appropriate for using them as raw materials in the production of industrially relevant compounds under solid-state fermentation (SSF) conditions. The main purpose of this project is to convert by SSF of some low cost/ abundant Romanian agro-industrial residues (apples and grapes) into value-added fungal metabolites, such as taxol (natural anticancer drug), and single-cell oils enriched with gama-linolenic acid (GLA) and carotenoids with individual and mixed cultures of an endophytic taxol-producing fungus (Colletotrichum capsici) and a fungus of the order Mucorales (for GLA and β-carotene). Moreover, the project has also proposed to determine the in vitro total antioxidant, antimicrobial and anticancer activities of the obtained microbial products.

NEW GENERATION OF INNOVATIVE FUNCTIONAL BEVERAGES WITH IMPACT ON HUMAN GASTROINTESTINAL HEALTH Call name: Joint Applied Research Projects - PCCA 2013 - call
PN-II-PT-PCCA-2013-4-0743 2014 - 2017

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO); B.W.B. S.R.L. (RO); INSTITUTUL REGIONAL DE GASTROENTEROLOGIE - HEPATOLOGIE PROF. DR. OCTAVIAN FODOR CLUJ-NAPOCA (RO); CENCIRA CENTRUL DE CERCETARE ȘI INOVARE AGROALIMENTARĂ SRL (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website: http://foodprofit.weebly.com/

Abstract:

In the last decades consumer demands in the field of food production has changed considerably. Consumers more and more believe that foods contribute directly to their health. Functional foods represent one of the most interesting areas of research and innovation in the food industry.

The aim of FOOD-PROFIT is to develop a new generation of probiotic functional beverages using agro-food waste, microencapsulated/granulated bacteria, bottling into controlled release bottles. Microcapsules/granules containing probiotics bacteria will be stored into bottle cap until consumption of beverage, when consumer must to push the bottle cap and the beads with bacteria or bacteria granules will be released into the juice mass. In vivo clinical investigations will be carried out in order to evaluate the beverages functionality on healthy human subjects and patients with gastrointestinal diseases. Thus, will be designed innovative functional probiotics beverages with impact on gastrointestinal health, increasing the innovation rate in this area, stimulating new beverage opportunities and taking away barriers for the transfer of knowledge to real innovation.

The strategic objectives of FOOD PROFIT project are: (a)Characterization of agro-food waste (apple, sugar beet, orange and grape) using advanced techniques (HPLC-MS, GC-MS, FTIR, UV-Vis) and cultivation, granulation, microencapsulation of probiotic bacteria (L. casei, L. plantarum, B. infantis, B. breve), (b)Development of different variants of beverages with microcapsules containing bacteria/ granulated bacteria and the optimal ratio between agro-food wastes (c) In vivo clinical investigation of probiotic beverages functionality and their effects on human gastrointestinal diseases.

FOOD-PROFIT is considered to be a strategic project for development of new concepts and products that, once transferred to the industry, will enable the European food industry to compete and take back the lead in the highly competitive global market.

FTIR and Raman spectroscopy as new molecular tools in the targeted action of nano and microstructures for diagnosis and molecular therapy of fibrosis. Call name: Postdoctoral Research Projects - PD-2012 call
PN-II-RU-PD-2012-3-0283 2013 - 2015

Role in this project: Project coordinator

Coordinating institution: PROPLANTA SRL

Project partners: PROPLANTA SRL (RO)

Affiliation: PROPLANTA SRL (RO)

Project website: http://pd283.bravesites.com

Abstract:

The aim of this study is to implement molecular specific spectroscopic methods like FTIR (Fourier-transform infrared absorption), Raman and SERS (Surface-enhanced Raman scattering) in order to monitor nano- and microstructures as potential markers for disease diagnosis and targeted molecular therapy. The ability to diagnose the early onset of disease, rapidly, non-invasively and unequivocally has multiple benefits, including the early intervention of therapeutic strategies. Microbubbles used currently in ultrasound imaging will be functionalized and used for the first time in conjunction with molecular specific methods like FTIR, Raman and SERS in order to monitor their interaction with fibroblast. The finality of the here proposed study is to monitor by molecular specific optical spectroscopic methods (FTIR, Raman/SERS), the targeted molecular therapy of fibrinolytic agent functionalized microbubbles, which have the property to destroy fibrosis (collagen fibers). Experiments will be preformed “in vitro” on fibroblast cell culture and “in vivo” on experimental mice subjects. This kind of analysis is of major impact in domains like pharmacology, as well as medical and drug research. The present proposal will be an advanced training in the multi-disciplinary area of chemical and medical biotechnology, leading to the fellow’s competence diversification and improvement of the prospects of reaching and reinforcing a position of professional maturity and independence.

Nanobiotechnology tools for molecular medicine applications: Nano- and microstructures synthesis, characterisation and in vitro studies Call name: Projects for Young Research Teams - TE-2011 call
PN-II-RU-TE-2011-3-0154 2012 - 2015

Role in this project: Key expert

Coordinating institution: Universitatea de Stiinte Agricole si Medicina Veterinara Cluj-Napoca

Project partners: Universitatea de Stiinte Agricole si Medicina Veterinara Cluj-Napoca (RO)

Affiliation:

Project website: http://www.usamvcluj.ro/nanopart-cell/

Abstract:

Recent advances in nanobiotechnology show great potential for nano- and microstructures to be used as drug carriers in targeted molecular therapies. There is a continuous need for developing new nanomaterials based on which one could build diagnosis and treatment nanosystems. The present project is focused on the synthesis, characterisation and in vitro testing of two different types of functionalised particles with the final purpose of using them as fibrinolitic agents, for potential applications in targeted molecular medicine: (a) functionalised gold nanoparticles (AuNPs) and (b) functionalised phospholipidic microbubbles. The project aims at synthesising the functionalised particles, characterising them by modern electron microscopy (scanning electron microscopy) and molecular spectroscopy techniques (Raman spectroscopy, surface enhanced Raman scattering, Fourier transform infrared spectroscopy), and performing experiments on fibroblast cell cultures to test the biocompatibility and the interaction of the obtained nanostructures with the cells. Understanding the cell-particle interaction is crucial when considering any potential applications. The proposed subject is of particular interest, since the use of functionalised particles in therapeutics and imaging and understanding their effect on cell functions are among the current challenges in nanobiotechnology and nanomedicine.

New approaches in the detection of chemical residues in complex matrices using TLC-SERS: melamine, antibiotics and pesticide detection in milk and milk powder Call name: Projects for Young Research Teams - TE-2010 call
PN-II-RU-TE-2010-0323 2010 - 2013

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BABES-BOLYAI DIN CLUJ-NAPOCA

Project partners: UNIVERSITATEA BABES-BOLYAI DIN CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA BABES-BOLYAI DIN CLUJ-NAPOCA (RO)

Project website: http://www.phys.ubbcluj.ro/~nicolae.leopold/pages/te323.html

Abstract:

Determination of chemical residues in complex matrices (e.g. food products), requires complex, laborious and complex analysis, involving the use of separation methods (CE, GC, HPLC) coupled with an efficient detection system (MS, MS-MS). This makes the current methodologies to be costly and time consuming. This project proposes a much easier approach, with comparable performances: thin layer chromatography (TLC) separation - ultrasensitive raman (surface-enhanced Raman scattering - SERS) detection. This innovative approach will be implemented for the detection of melamine, antibiotics and pesticides chemical residues in milk and milk powder, at detection limits of µg/kg. The sers detection method will be performed after an original own methodology, developed in our lab – in-situ photoreduction synthesis of the silver substrate, necessary for Raman amplification. Thus, the separated substances by tlc will be identified directly based on the sers spectral fingerprint. The tlc-sers approach shows advantages over the standard tlc-uv: sers spectroscopy provides a unic spectral fingerprint for each type of molecule, thus the use of standards is not required, and not at least, shows a higher detection sensitivity. The objectives of this project involve interdisciplinary activities, the methodology will be implemented using innovative and original methods, the applicability of TLC-SERS analysis for the determination of chemical residues can be extended to most food and environmental samples. Special attention will be given to human resource, developing the capacity for innovation and working with new technologies.

TESTING THE ANTIOXIDANT, ANTIPROLIFERATIVE AND PROAPOPTOTIC PROPERTIES OF BLUEBERRY POLYPHENOL EXTRACT ON TUMORAL CELL CULTURE Call name: Projects for Young Research Teams - TE-2010 call
PN-II-RU-TE-2010-0168 2010 - 2013

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA DIN CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA DIN CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA DIN CLUJ-NAPOCA (RO)

Project website: http://www.usamvcluj.ro/TE 186 AFINE/

Abstract:

IN OUR COUNTRY, BLACK BLUEBERRIES (VACCINIUM MYRTILLUS L.) GROW IN HILLS ACID AREAS (MAJORITY) BUT IS ALSO FOUND IN CULTURE. BESIDES THEIR WATER, SUCROSE, PROTEIN AND PECTIC SUBSTANCES CONTENT, BLUEBERRIES CONTAIN A HIGH CONCENTRATION OF ANTIOXIDANTS WHICH NEUTRALIZE FREE RADICALS RESPONSIBLE FOR THE PRODUCTION OF DEGENERATIVE DISEASES. DUE TO MULTIPLE BENEFICIAL EFFECTS ON HUMAN HEALTH OF BLUEBERRIES (IMPROVE EYESIGHT, PREVENT MACULAR DEGENERATION, ANTIDIABETIC AND ANTITUMOR PROPERTIES), THEY ARE CONSUMED FREQUENTLY IN NATURALLY FORM AND AS VARIOUS PREPARATIONS (SUPPLEMENTS NUTRITIONAL).

RESEARCH SHOWS THEIR CYTOTOXIC EFFECT (BY VIABILITY CELL) ON VARIOUS TUMOR CELLS: COLON, PROSTATE AND LIVER CELLS.

THERE IS NO LITERATURE DATA RELATING ON CYTOTOXIC EFFECT TO THE BLUEBERRY EXTRACT ON OVARIAN AND BREAST TUMOR CELLS.

THE MAIN PURPOSE OF THIS PROJECT IS TESTING THE ANTIOXIDANT EFFECT OF TOTAL POLYPHENOL EXTRACT AND THE ANTHOCYANINS EXTRACT AND THEIR PROAPOPTPTIC EFFECT ON OVARIAN AND BREAST TUMOR CELLS.

THIS WILL BE ACHIEVED BY FURTHER AIMS:

O1-THE EXTRACTION FLAVONOIDS BIOACTIVE COMPOUNDS AND EXTRACTS CHARACTERIZATION USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC (HPLC)

O2–DOSING THE BIOACTIVE COMPOUNDS: TOTAL POLYPHENOLS, TOTAL ANTHOCYANINS, TOTAL FLAVONOIDS

O3 - EVALUATION IN VITRO OF ANTIOXIDANT ACTIVITY FOR THE EXTRACTS USING ANTIOXIDANT METHODS

O4 - TESTING THE CYTOTOXIC EFFECTS OF THE TOTAL EXTRACTS AND ANTHOCYANIN FRACTION EXTRACT ON OVARIAN AND BREAST TUMOR CELLS.

O5-EVALUATION OF ANTIOXIDANT STATUS OF THE CELLS TREATED WITH BLUEBERRY EXTRACT

INNOVINC Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetatori
PN-III-P1-1.1-MC-2018-0453 2018 -

Role in this project: Project coordinator

Coordinating institution: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA

Project partners: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Affiliation: UNIVERSITATEA DE STIINTE AGRICOLE SI MEDICINA VETERINARA CLUJ-NAPOCA (RO)

Project website:

Abstract:

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List of research grants as project coordinator or partner team leader
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