BrainMap

Mihai Sorin David

CSII Dr.

Researcher - CENTRUL INTERNATIONAL DE BIODINAMICA

Researcher

Web of Science ResearcherID: not public

Expertise & keywords

Self-powered enzyme micropumps for microfluidic sensors Call name: P 4 - Proiecte de cercetare exploratorie - PCE-2021
PN-III-P4-PCE-2021-1231 2022 - 2024

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/SPEMS/spems.html

Abstract:

The last decades have witnessed an exponentially increasing interest in cost-effective, easy-to-use and portable diagnostic devices. Such devices very often integrate microfluidic elements. Core issues for such developments are that of bypassing the use of externally driven pumping (which is expensive and bulky) and that of the conversion of the measurements results in a response simple enough to be easily understood by a general user. Self-powered enzyme micropumps, which induce flows through biochemical reactions, have recently emerged as alternatives to externally driven pumping. However, the vast majority of enzyme micropumps reported so far have large lateral dimensions (≥ 1 mm). An in-depth theoretical analysis to quantitatively understand the flows generated by enzyme micropumps and to predict optimal micropump geometry is yet to be developed. Moreover, enzyme micropumps were up to now studied using microscopes which are costly and lack portability. Our project aims at addressing these issues by developing novel, self-powered enzyme pumps with small lateral dimensions (~ 80 µm) which can serve either as a component for microfluidic sensing devices or directly as sensor. We will also develop the theoretical models necessary to understand the topology and magnitude of the flows produced in the sensing devices. Finally, we will show that the developed, micropump-based sensing devices can be used in combination with the camera of a mobile phone.

Rapid Antibiotic Susceptibility Testing based on Drug Driven Bacterium Dynamics Call name: P 4 - Proiecte de cercetare exploratorie - PCE-2021
PN-III-P4-PCE-2021-1281 2022 - 2024

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/BacteriumDynAST/BacteriumDynAST.html

Abstract:

Non-invasive, label-free analysis of single cell dynamics, highly demanded by biomedical, biotechnological, biosafety and environmental applications, currently raises serious technological challenges. Founded on recent results and original research developments of the project leader and his team, BACTERIUMDynAST proposes label free time-lapse Electro-Optical microphysiology assessment of individual cell dynamics as a ground breaking concept in biosystems engineering and biohazard assessment. To advance and prove the concept, in the 33 months duration of the project, we address rapid Antimicrobial Susceptibility Testing (AST) challenges through an innovative design enabling physiomics data from hundreds of individual cells to be simultaneously monitored/assessed. BACTERIUMDynAST advanced control (capture and controlled focalization within the working distance of a high numerical aperture objective) of magnetically tagged target cells (e.g. bacteria), and AI assisted multiparametric data processing are seen as paramount in reaching the ultrafast phenotypic AST demands and the foreseen wide applicative impact.

BACTERIUMDynAST capability to provide cellular electro-optical images with high temporal and spatial resolution will facilitate creation of the next generation of (single)cell-based biosensing platforms likely to support breakthrough applications involving both natural cells (from pathogenic bacteria/fungi to circulating tumor ones) and synthetic cellular sentinels.

Development of a highly sensitive and selective SERS aptasensor for medical diagnosis Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-1998 2022 - 2024

Role in this project: Key expert

Coordinating institution: UNIVERSITATEA BABES BOLYAI

Project partners: UNIVERSITATEA BABES BOLYAI (RO); CENTRUL INTERNATIONAL DE BIODINAMICA (RO); INSTITUTUL NATIONAL DE CERCETARE DEZVOLTARE PENTRU TEHNOLOGII IZOTOPICE SI MOLECULARE I N C D T I M (RO)

Affiliation:

Project website: https://sites.google.com/view/nanoaptadia/home

Abstract:

Biomarkers are currently used for detection of diseases and monitoring therapeutic progresses in diagnostics. The accurate measurement of biomarkers in human patient samples is exceedingly important requirement for any analytical method. This project aims to develop an experimental demonstrator designed to provide pertinent solutions to three unmet medical needs in diagnostics: (1) high sensitivity; (2) high specificity and (3) label-free detection of disease biomarkers. Specifically, a novel biosensing nanotechnology will be implemented by coupling a new class of high affinity biorecognition elements called aptamers at the surface of plasmonic nanoplatforms recently developed in our laboratory. While the aptamers attached onto the metallic surface can specifically recognize the target molecules (biomarkers), the signal transduction will be based on ultrasensitive Surface Enhanced Raman Scattering (SERS). SERS has previously demonstrated its analytical performance toward “single-molecule sensitivity” and ability to identify and discern biomolecules by their spectral “Raman fingerprint” signature. By exploiting the complementary expertise of three research centers in SERS spectroscopy, chemistry and surface engineering, and fabrication and characterization of plasmonic nanoplatforms we expect to provide the feasibility of aptamer-modified SERS nanosensor for high sensitivity, high specificity and label-free detection of some relevant disease biomarkers. The as-fabricated “microchip” inserted in a Raman spectrometer to collect the SERS signal from the targeted biomarkers can offer unique characteristics, competitive advantages compared to other detection systems in the market and real potential for technology transfer.

Accurate sensing platform for VOC detection in air quality control systems of smart cities Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-3090 2022 - 2024

Role in this project: Project coordinator

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/AQVOC/AQVOC.html

Abstract:

Project AQVOC aims to improve the capabilities for air quality monitoring in smart city applications by development of a fast, sensitive and reliable device for detection of outdoor and indoor Volatile Organic Compounds (VOCs) based on the Magneto-Optical Surface Plasmon Resonance (MO-SPR). The validated demonstration model will be based on the SPR technique, magnetically augmented, towards a product that can be integrated in the market. The device will use available modular benchtop MOSPR equipment previously developed by our team combined with our portable SPR platform with imaging capabilities, patents pending. The sensitive area will consist in an array of spots of gas sensitive polymers and metallic oxides providing a specific “fingerprint” after interacting with individual VOCs. Data will be collected and analyzed using the Principal Component Analysis (PCA) approach to extract relevant data regarding the nature and quantity of the target VOC. The project starts from TRL2, and is expected to reach TRL 4 - the validation of the components and of the detection platform in the laboratory environment. Validating the platform will make an easier task for a future development towards integrating the whole setup into a complete model. After project completion, the AQVOC platform is intended to be further developed and checked to successively reach the next TRLs for being produced and reach the market. The project activities are sustained by a well-equipped infrastructure and a multidisciplinary team of researchers with expertise in all needed areas: modelling & data analysis, instrumentation and software development, thin film deposition and characterization, micro-fabrication.

Electrochemical assay for the rapid and sensitive detection of β-lactamase activity Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2021-2298 2022 - 2024

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/ELECTROLACTAM/electrolactam.pdf

Abstract:

Antimicrobial resistance is predicted to kill 2.4 million people in Europe, North America and Australia in the next 30 years and to cost up to 3.5 billion US dollars per year. The situation is especially worrying in Romania, where an average antimicrobial resistance prevalence rate of 42.6% of all infections, the highest in the EU, was reported in 2015. As a consequence, antimicrobial resistance has become a central issue at the top of the public health agenda of many countries around the world, including Romania. It was discovered that microbes can render β-lactam antibiotics ineffective by producing β-lactamases which are able to hydrolyze the β-lactam ring of such antibiotics. Therefore, there is a great interest in analytical methods which can detect the β-lactamase activity of bacteria while avoiding lengthy bacterial growth steps. In this context, the present project aims to develop a novel electrochemical assay that allows the sensitive (≤ 0.1 mU/mL) and rapid (≤ 30 min) detection of β-lactamase activity. The electrochemical assay will be suitable to measure the β-lactamase activity of bacteria after these are collected from clinical samples by immunomagnetic separation and before any bacterial growth step. Such an assay can contribute to reducing the time needed to treat patients with the appropriate antibiotic. However, during the present project we will approximate bacteria expressing β-lactamase with magnetic microspheres modified with β-lactamase (and a follow-up project is planned for taking the new assay to bacteria level). Four work packages will take the concept to a Technology Readiness Level of 3. Project feasibility is assured by the expertise of the project team, the relevant background knowledge produced by the project team, and the good infrastructure existing at the host institution. Dissemination and IP protection activities will also be carried out in order to increase the impact of the project.

Electrical field modulated aptasensors for proteins: sensitivity, selectivity and control of enzyme activity Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-2297 2021 - 2024

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/E-MAP/e-map.html

Abstract:

The project has at core the modulation of aptamers’ conformation by an electric field (EF) to control their immobilization and the binding of specific ligands. Two main objectives are set: (1) the pursuit of highly sensitive detection, demonstrated with an electrochemical aptasensor for β-2-microglobulin, the protein linked to dialysis–related amyloidosis and (2) a systematic investigation of the aptamer-lysozyme binding interface to reveal how the EF affects the binding selectivity and the enzymatic activity of the bound protein. The study focuses, respectively on: (i) the detection of soluble protein aggregates by coupled electrochemical-surface plasmon resonance (EC-SPR) and chemometrics, aimed at the pattern recognition of the aggregation in biopharmaceuticals; (ii) the controlled binding/release of lysozyme relevant for biocatalysis and the food industry where the enzyme is used as an antimicrobial agent. Significant investigative effort by EC-SPR, electrochemical-surface enhanced Raman spectroscopy, nanoimpact electrochemistry, binding studies with lysozyme mutants and chemometric analysis of the data will unravel correlations with the observed changes in the enzymatic activity in the EF. The project will strengthen collaborations with high expertise groups from Romania and the USA, open new research avenues, promote the scientific competitiveness of the team and develop the research infrastructure of the host institution, including by attracting young team members.

Multiplexed electro-plasmonic system with high sensitivity and specificity for analyte detection in real samples Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-1433 2021 - 2023

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/MEPS/meps.html

Abstract:

MEPS addresses the unmet needs of bioanalysis market for analytical means with increased sensitivity and robustness by developing a label-free, very sensitive and specific multisensing electro-plasmonic system, based on surface plasmon resonance (SPR) measurements, for simultaneous detection of multiple analytes in real samples. Therefore, a novel and more sensitive way to measure plasmonic based electrochemical impedance by angle-resolved SPR phase assays in a common-path interferometry scheme will be advanced. The setup will comprise a multiplexed phase SPR system to be developed using ICB proprietary technology and a high speed synchronized acquisition and processing system based on a CMOS sensor (~30000 fps), combined with a signal generator and microfluidics. A biomedical application will demonstrate system capabilities i.e. simultaneous and sensitive detection in undiluted serum of several cardiac biomarkers with key role in diagnosis, risk assessment, treatment, and supervision of cardiovascular diseases. As a low-cost and robust alternative to antibodies, aptamers will be used as biorecognition molecules. The sensor surface will be functionalized with a suitable layer for improved specific detection and reduced nonspecific response. The system will be validated by conventional methods. Due to its novelty and analytical relevance for label-free detection in real samples, the proposed system and aptasensors promise significant both scientific and socio-economic impact.

High resolution multiparametric dynamics at single cell level: virus detection by assessing cellular response to viral exposure Call name: P 4 - Proiecte de Cercetare Exploratorie, 2020
PN-III-P4-ID-PCE-2020-2432 2021 - 2023

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/DYNASCOPE/dynascope.html

Abstract:

Cellular-based sensing platforms face serious challenges before enabling full realization of the concept of “cell sentinels” against biological threats (in particular against viral infections, as stringently called into action given the SARS Covid 2 pandemics).

Grounded on the PL expertise and the resources of the International Centre of Biodynamics team, DynaScope advances a sensing platform for quantification of viral (particles) entry in model cells and characterisation of cellular dynamics associated with viral replication progress. The biosensing concept is based on label-free monitoring the dynamics of electrical, morphological, structural and metabolic properties of relevant intracellular structures upon viral exposure, at high spatial and temporal resolution. We achieve this through: (a) multiplexed/multiparametric assays, (b) use of bioengineered cells (c) analysis of time-lapse dynamical data. Via a broad biotechnology/ bioengineering effort we integrate phase (via SPR/QPI) and electrical impedance information within an AC electric field actuated plasmonic based EIS (P-EIS) microscopy setup. The envisaged platform will provide accurate, dynamic electro-optical (quantitative phase) maps of cellular changes corresponding to physiological states of both single cells (ACE2 HEK modified cells) and cell ensembles. The unique detection characteristics of the DynaScope are essential for applications in the fields of cell signalling, drug screening and hazard evaluation.

Portable biosensor coupled with a sampling drone for the in situ assessment of seawater toxicity Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-MARTERA-MOBILTOX-1 2021 - 2023

Role in this project:

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); UMR CNRS 6144 GEPEA Equipe CBAC Université de Nantes (FR); Heliceo (FR)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/MOBILTOX/mobiltox.html

Abstract:

The preservation of the oceans is a major issue of the 21st century. In this context, the European Union is committed to protecting our seas and oceans, as indicated in the Marine Strategy Framework Directive. Despite this, there is today a significant lack of methods aimed at qualifying its environments, and in particular with regard to the impact of pollutants on marine ecosystems. The aim of MOBILTOX project is to contribute to this effort by providing a mobile platform for the in situ assessment of water toxicity as an early-warning system. The platform will combine two analysis complementary modules (biosensors) relying on biological indicators and a sampling drone. The first sensing module is based on whole microbial cells as indicator of overall toxicity (approach based on inhibition of respiratory activity- fluorescent sensors). It will be used to determine the toxicity level caused by the pollutants mixtures in the studied environments (harbours, coastal areas, etc.). This first analysis level will provide information about the overall quality of the environment (the aim is not to detect specific toxic compounds). The choice of the biological indicator is crucial, therefore the cells will be isolated from the targeted environments (coastal area, harbors, mouth of rivers such as the Danube (Romania) or the Loire (France) in order to ensure a good representativeness of the information collected by this first approach. The second module aims to detect specific groups of contaminants, i.e. inhibitors of photosynthesis such as herbicides and metals (main marine pollutants from run-off from agricultural land and industrial activities) via inhibition of photosystem II (PSII) immobilized on electrodes.

The sampling platform will be developped from an existing drone suitable for a deployement in these coastal environments (resistance to wind, waves,etc.) and equipped with a set of probes (pH, temperature,etc.). A sampling module will be integrated on the drone and the whole will be controllable remotely from the shore. The mobile platform prototype will be evaluated in harbors and coastal areas close to agricultural and river discharge zones in the Black Sea and the Atlantic and compared with standard chemical and ecotoxicity tests. A set of robust microbial strains isolated from marine environments and sensitive PSII complexes from marine phototrophic microorganisms are key project results facilitating further development of MOBILTOX in a commercial product.

Core integration of novel functional, adaptive materials into a smart, highly sensitive analytical system for point of need environmental applications Call name: P 3 - SP 3.2 - Proiecte ERA.NET
ERANET-M-SmartMatter 2020 - 2023

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); Consiglio Nazionale delle Ricerche - Istituto per la Tecnologia delle Membrane (IT); Centre National de la Recherche Scientifique (FR); Centro Analisi Biochimiche Sas (IT)

Affiliation:

Project website: https://www.biodyn.ro/PROJECTS/SmartMatter/smartmatter.html

Abstract:

SmartMatter proposes novel adaptive functional materials featuring magneto-plasmonic properties and validated capability for boosting the detection sensitivity of greenhouse gases and low molecular weight compounds (LMWC). Three research institutes and two SMEs from Romania, Italy and France gather their expertise to achieve breakthrough adaptive 3D nano platforms - nanoparticle/dynameric conjugates – to enhance the analytic response of (magneto)-optical waveguides sensing chips and of the analyte specific interaction sites, identified as bottlenecks in reaching the analytic potential of portable, autonomous sensing devices.

The customized design of novel materials based on nanoparticle/dynameric conjugates envisaged by SmartMatter aims at achieving extreme sensitivities via innovative exploitation of the known high dependency of the analytic behavior of optical waveguide sensors (e.g. Surface Plasmon Resonance, SPR and Magneto Optic SPR, MOSPR) on the inner structure of the sensing chip. SmartMatter sensors become active, dynamic amplifiers of the target specific affine reactions that modulate their structure. This approach goes beyond the state of the art SPR type sensors, with fixed structure that passively transduce affinity reactions taking place above their surface.

Moreover, SmartMatter proposes a leap forward, warranted by the use of 3D dynameric reservoirs to amplify the sensitivity for LMWC, notoriously low in classical assays. Besides their role as framework for the optical waveguide (SPR & MOSPR), they will increase the absorption of small molecules in 3D multilayer dynamer-nanoparticles conjugates, to adaptively improve the accumulation of the analyte and thus further enhance the sensitivity of the assay for small molecules. In achieving this, the classical use of the affinity sensing platforms will be replaced by a constitutional strategy: the dynamic self-organization of components within systems will be controlled for exploiting their adaptive behavior toward the fittest binding structure to the affine compound, largest conformational change and accurate control of the interaction responses.

By combining the concept of interactive dynameric matrices/ networks with natural biocatalysts, and nanoparticles we provide a facile in situ strategy to adaptively improve the accumulation of the analyte and achieve bio-catalytic activity.

As a breakthrough, nanoparticles within the 3D nanoplatforms are set to play a double role: as framework for optical waveguide and as sites for interaction with the analytes therefore developments will be harmonised with tuning of the functionalization of nanoparticles with bioactive molecules such as enzymes, abzymes, aptamers able to interact with target gases and LMWC.

SmartMatter will achieve integration in a point of need smartphone based optical wave-guide analytical device, its testing and validation in a laboratory environment against greenhouse gases and LMWC for harvesting the full capabilities of the developed smart materials.

The project starts at TRL2 to successively reach TRL3 and TRL4, at its completion. An industrial advisory board will be established to facilitate further development after the end of the project and the transition of the technology to higher TRLs.

The system will be portable, have monitoring and interconnectivity capabilities thus, it is expected that in a subsequent step it will reinforce the achievement of the European strategic policy targets in terms of reduction of greenhouse gas emission by providing a flexible network of sentinel sensors. Wider impact is foreseeable for biomedicine, chemistry and energy fields where the new materials design warrants enhanced catalytic power, improved sensitivity and specificity and compatibility with portable formats. The forefront research merging nanosensors, nanomaterials and dynamers, the dissemination and training components of the project will ensure strengthened innovation excellence of the European research institutes both within and outside the project consortium, will contribute to improved competitiveness and strengthened industrial leadership (upon reaching market valorisation).

Automated Analytical Platform for Rapid Antimicrobial Susceptibility Testing Call name:
PN-III-P2-2.1-PED-2019-4932 2020 - 2022

Role in this project: Project coordinator

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA ()

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA ()

Project website:

Abstract:

The scope of AutoAST project is the development and validation of an Automated Analytical Platform for Antimicrobial Susceptibility Testing of microbes in clinical samples.

The project aim is to evaluate the effect of the antibiotics on the target bacteria by exposing the aggregates containing target bacteria captured by magneto-immuno separation

to antibiotics and measure their electrical properties; changes in the electrical properties for samples which are exposed to a set of different antibiotics will be used to determine

the antimicrobial susceptibility of the target bacteria. The projects aims to accomplish the following objectives: (1) Development of an analysis platform for automatic evaluation

of multiple antibiotics – AutoAST platform, (2) Evaluate the effect of the antibiotics to immuno-magnetically captured bacterial cells (3) Develop the measurement protocol involving

multiple antibiotic testing and (4) Validate the AutoAST platform with respect to representativeness, reproducibility and repeatability to evaluate the effect of the antibiotics bacteria

present in a sample. Project objectives are in line with envisaged outcomes and its feasibility is reinforced by the expertise of the AutoAST team covering the areas to be approached

from modelling, data analysis and software development to instrumentation, micro/nanofabrication and microbiology. Following AutoAST project accomplishment the analysis platform & measuring protocols

are expected to be subsequently involved in tests, demonstrations and validations in relevant environments and create the premises for the future development of the platform at higher TRLs,

for successively reaching TRL5, TRL6, TRL7, TRL8 and TRL9, to be further produced and reach the market.

Automated Analytical Platform for Rapid Antimicrobial Susceptibility Testing Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-4932 2020 - 2022

Role in this project: Project coordinator

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: https://www.biodyn.ro/PROJECTS/AutoAST/autoast.html

Abstract:

The scope of the proposed project is the development and validation of an Automated Analytical Platform for Rapid Antimicrobial Susceptibility Testing (

Rapid, quantitative identification of microorganisms in a lab-chip assay Call name: P 2 - SP 2.1 - Proiect experimental - demonstrativ
PN-III-P2-2.1-PED-2019-5185 2020 - 2022

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: https://www.biodyn.ro/PROJECTS/BactoID/bactoid.html

Abstract:

The development of methods for the rapid identification of microorganisms is a major step towards efficient biotechnology products, food and water safety control and accelerated clinical diagnosis of infectious diseases. Despite significant efforts in the development of new analytic tools for pathogenic cells assays (aimed for identification, enumeration and rapid phenotyping), cell cultivation on selective solid media remains the gold standard in microbiological analysis. BactoID proposes the development, testing and validation of an experimental prototype of a novel multiparametric electro-optic platform with microarrays allowing analysis of chromogenic spots as an effective tool for rapid, sensitive and specific identification of microorganisms, translatable in bench top and portable formats. BactoID aims to shrink the time lag of colony identification on solid media, typical in classical microbiological analyses by integrating affinity magneto capture and magnetic guidance at microscale, with arrays of selective and differential chromogenic media in a lab-chip format compatible with multiparametric, high resolution electro-optic assays.

BactoID project harnesses the available knowhow and technological resources within the International Centre of Biodynamics (ICB) and proposes to substantially advance the ICB innovative research concerning a) detection and quantitation of target analytes, b) the lab-on-chip approaches for multimodal modification and evaluation at microscale, in synergy with other validated platforms within ICB. BactoID platform will allow rapid, sensitive identification of pathogenic bacteria, validated in laboratory on selected microorganisms (chosen, without restricting the applicability to other domains, for enhanced relevance for the overarching fields of Biotechnology and Medicine) upon successful completion of the project.

Mobilitate cercetător Mihai Sorin David Call name: P 1 - SP 1.1 - Proiecte de mobilitate pentru cercetatori
PN-III-P1-1.1-MC-2020-0018 2020 - 2020

Role in this project: Project coordinator

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation:

Project website:

Abstract:

Single-molecule detection of DNA hybridization, based on electrochemical surface plasmon resonance microscopy and magnetic tweezers Call name: Projects for Young Research Teams - RUTE -2014 call
PN-II-RU-TE-2014-4-2363 2015 - 2017

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/PROJECTS/ESPRIM/esprim.html

Abstract:

We propose a new method for single-molecule detection of DNA hybridization, based on Electrochemical Surface Plasmon Resonance Microscopy (ESPRM) and magnetic tweezers, with electro-optical monitoring of periodic oscillations exhibited by a single DNA-magnetic bead (MB) complex in variable magnetic field gradient. The concept of this project exploits the difference between the elastic behavior of single strand and double strand DNA at single molecule level. The proposed method overcomes the spatial limitations of the evanescent field i.e. the exponential decay with distance of the internal reflection fluorescence microscopy (TIRFM) coverage, allowing, for the first time, ultra-sensitive assessment of longer DNA strands (hundreds of bases). Moreover, the unified platform capable of simultaneous electro-optical monitoring, with integrated microfluidics and magnetic actuation will be suited for DNA based biosensing in biomedical and environmental applications. Taking into account the interdisciplinary nature of the project, the project leader will advance her expertise in several fields: atomic force microscopy (AFM), surface chemistry, numerical simulations of magnetic field fostering controlled actuation of MB, data analysis, ESPRM, microfluidics and LabView programming.

Electro-Plasmonics for the analysis of the dynamics of cellular processes and biomolecular interactions - BioScope Call name: Complex Exploratory Research Projects - PCCE-2011 call
PN-II-ID-PCCE-2011-2-0075 2012 - 2016

Role in this project: Key expert

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO); SC INSTITUTUL DE OPTOELECTRONICA SA (RO); CENTRUL INTERNATIONAL DE BIODINAMICA (RO); Universitatea din Bucuresti (RO); CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/php/biodyn.php?act=Projects&idm=5&idsm=11&locale=en

Abstract:

Whereas experiments on processes at single cell level are highly demanded by biomedical industry they currently raise serious technical challenges. Therefore, BioScope project advances the State of the Art by proposing creation of the Electro-Plasmonic Analysis System (EPAS), an innovative engineered system integrating advanced optics and electronics that will reveal the dynamics of electrical and morphological properties of relevant intracellular structures and biointerfaces with an unprecedented temporal (1 ms) and spatial (200 nm) resolution.

A multidisciplinary consortium will develop EPAS by harnessing Surface Plasmon Resonance, SPR, Magnetism (to increase SPR and thus overall sensitivity), Optogenetics (to control cell properties through light for calibration purposes), Modelling (to reveal details of how cells in electric field affect SPR), Bioinformatics (for the analysis of data) and Biochemistry (to validate/substantiate the results).

Novel analytic capabilities of EPAS to perform fast, noninvasively and label free, thorough in situ measurements for gaining new insights into cellular processes (such as changes in adherence, conformation, and organelle distribution, swelling / shrinking, exo- and endocytosis, neurotransmitter release, electrical spiking, etc.) at single cell level will be explored.

BioScope results will facilitate creation of the next generation of (single)cell-based analytical platforms likely to support breakthrough applications spanning from quality control of food and aquatic media to bio-medical ones coping with analysis of interaction mechanisms between selected cells and various stimuli (including those fostering new pharmacological products), cell assays as alternatives to animal (preclinical) testing, or last but not least, supporting cutting-edge cell-based “Disease in a Dish” approaches.

Sensitive quantitation of target microorganisms using dual electro plasmonic analysis and magnetic actuation Call name: Postdoctoral Research Projects - PD-2012 call
PN-II-RU-PD-2012-3-0467 2013 - 2015

Role in this project: Project coordinator

Coordinating institution: CENTRUL INTERNATIONAL DE BIODINAMICA

Project partners: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Affiliation: CENTRUL INTERNATIONAL DE BIODINAMICA (RO)

Project website: http://www.biodyn.ro/PROJECTS/Contract%20No.%2082-30.04.2013/Contract%20No.%2082-30.04.2013.html

Abstract:

The aim of this project is the development of a method for fast detection of microorganisms in liquid samples with increased sensitivity by advancing dual SPR-EIS analysis in conjunction with periodic (magnetic) actuation. The concept is envisaged to overcome the limits of current methods regarding response time, sensitivity and robustness by capturing the target cells with modified magnetic beads (MB) and using specially designed sensing chips.

To this purpose the following objectives are envisaged:

1. Controlled capture of the analyte close to the sensitive area of the sensor chip.

2. Develop a sensitive analytical platform based on dual SPR & EIS assays and on periodic magnetic actuation for detection of microorganisms (case study on Escherichia coli O157:H7); establish the detection limits and reproducibility

3. Validation of results (using a luminescence method).

Project results are expected to have a wide applicative impact by providing effective means to improve the existing approaches for detection and quantitation of microorganisms with applications in quality control of environment and food, as well as in bio-medicine.

The project activities will also support strengthening of Project Leader scientific carrier by acquiring knowhow on advanced modelling and data analysis, microfabrication, as well as by mastering SPR and EIS techniques.

Tracing proteins through food processing with biosensors Call name: Projects for Young Research Teams - TE-2011 call
PN-II-RU-TE-2011-3-0302 2011 - 2014

Role in this project:

Coordinating institution: Centrul International de Biodinamica

Project partners: Centrul International de Biodinamica (RO)

Affiliation:

Project website: http://www.biodyn.ro/PROJECTS/Contract%20No.%2006-05.10.2012/urmarirea%20proteinelor.pdf

Abstract:

The overall objective of the project is to explore the possibility of tracing proteins through food processing using biosensors, responding to a real societal need originating from current regulatory and food industry requirements defining maximum admissible limits of allergen protein in food. Three main goals around which the activity of a new, young research team will be centered will be put forward in an integrated analytical platform: Develop new biosensors for sensitive and specific detection of allergen proteins; to assess protein interactions in food matrices; demonstrate the feasibility of developed methods/sensors for tracing proteins through food processing by three practical applications relevant for food industry. Comparison of proposed sensors and procedures with current reference methods is envisaged for each of the three practical applications. The project fosters novel biosensors and working protocols related to: a simplified, sensitive detection method for allergen proteins, contributing to public safety and food quality control (a) and a new platform for studying protein interaction, aimed to study the correlation between changes in the conformation of a specific protein and parameters related to food formulation or manufacturing (b). At the end of the project, it is expected that a strong team will be consolidated and will acquire momentum to attract new funding and participate in larger research projects.

Sensing using the electrochemically-triggered motion of catalytic nanomotors (SENSMOTION) Call name: Projects for Young Research Teams - TE-2011 call
PN-II-RU-TE-2011-3-0237 2011 - 2014

Role in this project:

Coordinating institution: Centrul International de Biodinamica

Project partners: Centrul International de Biodinamica (RO)

Affiliation:

Project website: http://www.biodyn.ro/PROJECTS/SENSMOTION/sensmotion%20prez.pdf

Abstract:

Catalytic nanomotors are nanometer-sized objects which turn chemical reactions occurring at their surface into movement when suspended into solution. Although greeted with high expectations at their appearance, such catalytic nanomotors have seldom found applications. Therefore, SENSMOTION is set out to develop novel catalytic nanomotors which can be used for sensing low concentrations of reactive oxygen species (ROS) with increased temporal and spatial resolution. The following advances are proposed relative to the state of the art:

1.) Catalytic nanomotors which propel themselves in the presence of low concentrations of superoxide and hydrogen peroxide; Determination of the concentration of the selected ROS in the sample will be possible by recording the velocity of the nanomotors.

2.) An electrochemical method to record changes in nanomotor velocity, which can advantageously complete the commonly used analysis of optical images;

3.) A thorough understanding of the mechanism behind the motion of the novel catalytic nanomotors;

4.) A microarray of oxidases that can be used in combination with the developed nanomotors to sense the substrates of the oxidases; Detection of the substrates will be possible because oxidases use molecular oxygen as electron acceptor and produce hydrogen peroxide and / or superoxide in the presence of their main substrate.

While pursuing the ambitious research goals of SENSMOTION, a young research team will be assembled and will gain maturity.

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List of research grants as project coordinator or partner team leader	Download (58.75 kb) 20/05/2020
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